Expression data from human embryonic stem cells, early stage embryoid bodies, and hES-derived blast cells.
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(Redirected from GSE8884)
| PubMed ID: | none |
|---|---|
| Contact: | Jennifer A Hipp
|
| Species: | Homo sapiens |
| Platform: | Affymetrix GeneChip Human Genome U133 Plus 2.0 Array |
| Number of Samples: | 6 |
| Date Range: | 03/28/2006 - 09/29/2006 |
| Experiment Quality: | 95.2 7.37 % |
| Link to Repository: | GSE8884 |
| Original Intensity Data: | Download |
| Clean Intensity Data: | Download |
| Original PLIER Data: | Download |
| Clean PLIER Data: | Download |
| Clean CEL Files: | |
| Data Rank: | 
|
To understand the differentiation process of embryonic stem cells into hemangioblasts, gene expression profiles of ES, EB and Blast cells (BL) were analyzed.
Design: Total RNA was isolated from purified BC cells, day3.5-EBs and undifferentiated ESCs (from two hESC lines, H1 and H9) using the Qiagen RNAeasy kit We sought to obtain homogeneous populations of cells at each developmental stage in order to increase the purity of each sample.
Type: time course
Contents |
[edit] Contributors
- Jennifer Hipp
- Shi J Lu
[edit] Protocols
[edit] References
[edit] Links
GEO Accession for this experiment: GSE8884. (Public on Aug 27 2007)
RAW data: tar file.
[edit] Analysis
 Before Normalization |
 After Normalization & Cleaning |
[edit] Samples/Quality Control
| Sample | Date Scanned | Source Link | Probe Variance Data | Artifact Mask | Quality Score |
|---|---|---|---|---|---|
| H1 embryonic stem cells - GFP | 09/29/06 11:25:19 | GSM225042 | 
| 
| 96.2035 1.61 % |
| H1 embryoid bodies - GFP | 03/28/06 12:47:30 | GSM225043 | 
| 
| 96.8715 0.42 % |
| H1 blast cells - GFP | 03/28/06 13:03:56 | GSM225044 | 
| 
| 91.3046 42.08 % |
| H9 embryonic stem cells | 05/12/06 12:06:23 | GSM225045 | 
| 
| 93.8703 0.06 % |
| H9 embryoid bodies | 05/12/06 12:19:38 | GSM225046 | 
| 
| 98.1993 0.05 % |
| H9 blast cells | 05/12/06 12:32:21 | GSM225047 | 
| 
| 94.753 0.03 % |
