Comparison of splenic and small intestine lamina propria macrophages
From ArrayWiki
| PubMed ID: | none |
|---|---|
| Contact: | Tim Denning no email given |
| Species: | Mus musculus |
| Platform: | Affymetrix GeneChip Mouse Genome 430 2.0 Array |
| Number of Samples: | 4 |
| Date Range: | 05/05/2006 - 08/16/2006 |
| Experiment Quality: | 96.46 9.98 % |
| Link to Repository: | GSE8868 |
| Original Intensity Data: | Download |
| Clean Intensity Data: | Download |
| Original PLIER Data: | Download |
| Clean PLIER Data: | Download |
| Clean CEL Files: | |
| Data Rank: | 
|
The intestinal immune system must elicit robust immunity against harmful pathogens but restrain immune responses directed against commensal microbes and dietary antigens. The mechanisms that maintain this dichotomy are poorly understood. Here we describe a population of CD11b+F4/80+CD11c– macrophages in the lamina propria (LP) that express several anti-inflammatory molecules including interleukin 10 (IL-10), but little or no pro-inflammatory cytokines, even upon stimulation with Toll-like receptor (TLR) ligands. These macrophages induced, in a manner dependent on IL-10, retinoic acid and exogenous transforming growth factor-β, differentiation of FoxP3+ regulatory T cells. In contrast, LP CD11b+ dendritic cells elicited IL-17 production. This IL-17 production was suppressed by LP macrophages, indicating that a dynamic interplay between these subsets may influence the balance between immune activation and tolerance.
Splenic or small intestine lamina propria CD11b+11c- cells were isolated for RNA extraction and hybridization on Affymetrix microarrays. We sought to determine the unique genetic profile of small intestine lamina propria CD11b+11c- cells.
Design: 4 samples analyzed, 2 spleen and 2 intestine
Type: cell type comparison
Contents |
[edit] Contributors
- Bali Pulendran
- Tim Denning
[edit] Protocols
[edit] References
[edit] Links
GEO Accession for this experiment: GSE8868. (Public on Aug 24 2007)
RAW data: tar file.
[edit] Analysis
 Before Normalization |
 After Normalization & Cleaning |
[edit] Samples/Quality Control
| Sample | Date Scanned | Source Link | Probe Variance Data | Artifact Mask | Quality Score |
|---|---|---|---|---|---|
| Spleen CD11b(+)11c(-)_BP03 | 05/05/06 11:58:44 | GSM224733 | 
| 
| 94.6195 1.92 % |
| Intestine CD11b(+)11c(-)_BP04 | 05/05/06 11:38:08 | GSM224734 | 
| 
| 97.037 0.16 % |
| Spleen CD11b(+)11c(-)_BP20 | 08/16/06 12:04:55 | GSM224735 | 
| 
| 96.868 3.14 % |
| Intestine CD11b(+)11c(-)_BP21 | 08/16/06 12:14:08 | GSM224736 | 
| 
| 97.2989 34.69 % |
