MRNA expression in TTHB211 deletion mutant of Thermus thermophilus HB8 strain grown on a rich medium
From ArrayWiki
| PubMed ID: | none |
|---|---|
| Contact: | Akeo Shinkai
|
| Species: | Thermus thermophilus HB8 |
| Platform: | Riken Thermus thermophilus HB8 4K mRNA array |
| Number of Samples: | 6 |
| Date Range: | 06/24/2004 - 06/28/2005 |
| Experiment Quality: | UF: 99.04 AC: 5.71 % 96.67 |
| Link to Repository: | GSE8780 |
| Original Intensity Data: | Download |
| Clean Intensity Data: | Download |
| Original PLIER Data: | Download |
| Clean PLIER Data: | Download |
| Clean CEL Files: | Download |
| Data Rank: | 
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We observed the expression profile of the total mRNA of TTHB211 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC compared with that of wild type.
Design: Three wild-type and mutant T. thermophilus HB8 strains were each pre-cultured at 70oC for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70oC. Cells were collected after 8 h, and then crude RNA was extracted. In order to compare mRNA expression in the TTHB211 deletion mutant with that of wild type, the expression of each mRNA at the time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.
Type: cell type comparison
Contents |
[edit] Contributors
- Akeo Shinkai
- Aiko Kashihara
- Seiki Kuramitsu
- Shigeyuki Yokoyama
[edit] Protocols
[edit] References
[edit] Links
GEO Accession for this experiment: GSE8780. (Public on Sep 04 2007)
RAW data: tar file.
[edit] Analysis
 Before Normalization |
 After Normalization & Cleaning |
[edit] Samples/Quality Control
| Sample | Date Scanned | Source Link | Probe Variance Data | Artifact Mask | Quality Score |
|---|---|---|---|---|---|
| wild-type strain_480_2 | 06/24/04 12:15:09 | GSM172548 | 
| 
| UF: 98.9027 AC: 22.7 % 88.1 |
| wild-type strain_8h_1 | 06/28/05 11:28:40 | GSM172567 | 
| 
| UF: 98.8059 AC: 0.03 % 99.39 |
| wild-type strain_8h_2 | 06/28/05 11:40:23 | GSM172568 | 
| 
| UF: 98.981 AC: 0.23 % 99.38 |
| TTHB211 deletion mutant strain_8h_1 | 12/28/04 11:28:08 | GSM218190 | 
| 
| UF: 99.204 AC: 10.99 % 94.11 |
| TTHB211 deletion mutant strain_8h_2 | 12/28/04 11:33:59 | GSM218191 | 
| 
| UF: 99.2903 AC: 0.25 % 99.52 |
| TTHB211 deletion mutant strain_8h_3 | 12/28/04 11:39:53 | GSM218192 | 
| 
| UF: 99.0465 AC: 0.1 % 99.47 |
