The effects of chimeric EWS/ETS protein in human mesenchymal progenitor cells (MPCs).

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PubMed ID: none
Contact: Yoshitaka Miyagawa

Image:email_GSE8665.png
National Research Institute for Child Health and Development

Species: Homo sapiens
Platform: Affymetrix GeneChip Human Genome U133 Plus 2.0 Array
Number of Samples: 14
Date Range: 05/18/2006 - 10/10/2006
Experiment Quality: 97.15
0.29 %
Link to Repository: GSE8665
Original Intensity Data: Download
Clean Intensity Data: Download
Original PLIER Data: Download
Clean PLIER Data: Download
Clean CEL Files:
Data Rank:

We have examined the biological effect of EWS/ETS in human MPCs using UET-13 cells that are obtained by prolonging the lifespan of human bone marrow stromal cells using the retroviral transgenes hTERT and E7. By exploiting tetracycline-inducible systems for expressing EWS/ETS (EWS/FLI1 and EWS/ERG), we investigated candidates for genes whose expression is regulated by EWS/ETS in human MPCs.

Design: A change of expression profiles in the presence of EWS/ETS using Affymetrix HG-U133 Plus 2.0 (GPL570).

Type: Time course


Contents

[edit] Contributors

  • Yoshitaka Miyagawa
  • Hajime Okita
  • Hideki Nakaijima
  • Yasuomi Horiuchi
  • Ban Sato
  • Tomoko Taguchi
  • Yohko U Katagiri
  • Junichiro Fujimoto
  • Jun-ichi Hata
  • Akihiro Umezawa
  • Nobutaka Kiyokawa

[edit] Protocols

[edit] References

[edit] Links

GEO Accession for this experiment: GSE8665. (Public on Aug 03 2007)

RAW data: tar file.

[edit] Analysis

Gel Plots

Before Normalization

After Normalization & Cleaning

[edit] Samples/Quality Control

Sample Date Scanned Source Link Probe Variance Data Artifact Mask Quality Score
UET-13TR-EWS/FLI1 0hr 05/18/2006 12:38:44 GSM214917 Probe variance for UET-13TR-EWS/FLI1 0hr Artifact mask for UET-13TR-EWS/FLI1 0hr 95.4880
0.40 %
UET-13TR-EWS/FLI1 24hr 05/24/2006 13:12:19 GSM214918 Probe variance for UET-13TR-EWS/FLI1 24hr Artifact mask for UET-13TR-EWS/FLI1 24hr 96.2389
0.03 %
UET-13TR-EWS/FLI1 48hr 05/24/2006 13:24:33 GSM214919 Probe variance for UET-13TR-EWS/FLI1 48hr Artifact mask for UET-13TR-EWS/FLI1 48hr 96.5847
0.27 %
UET-13TR-EWS/FLI1 72hr 05/24/2006 13:40:12 GSM214920 Probe variance for UET-13TR-EWS/FLI1 72hr Artifact mask for UET-13TR-EWS/FLI1 72hr 96.2748
0.72 %
UET-13TR-EWS/FLI1 24hr tet+ 05/18/2006 12:54:48 GSM214921 Probe variance for UET-13TR-EWS/FLI1 24hr tet+ Artifact mask for UET-13TR-EWS/FLI1 24hr tet+ 97.1534
1.04 %
UET-13TR-EWS/FLI1 48hr tet+ 05/18/2006 13:10:26 GSM214922 Probe variance for UET-13TR-EWS/FLI1 48hr tet+ Artifact mask for UET-13TR-EWS/FLI1 48hr tet+ 97.2828
0.03 %
UET-13TR-EWS/FLI1 72hr tet+ 05/18/2006 13:33:03 GSM214923 Probe variance for UET-13TR-EWS/FLI1 72hr tet+ Artifact mask for UET-13TR-EWS/FLI1 72hr tet+ 97.8391
0.51 %
UET-13TR-EWS/ERG 0hr 10/02/2006 14:16:12 GSM214924 Probe variance for UET-13TR-EWS/ERG 0hr Artifact mask for UET-13TR-EWS/ERG 0hr 96.9629
0.35 %
UET-13TR-EWS/ERG 24hr 10/10/2006 15:03:10 GSM214925 Probe variance for UET-13TR-EWS/ERG 24hr Artifact mask for UET-13TR-EWS/ERG 24hr 97.8575
0.03 %
UET-13TR-EWS/ERG 48hr 10/10/2006 15:16:28 GSM214926 Probe variance for UET-13TR-EWS/ERG 48hr Artifact mask for UET-13TR-EWS/ERG 48hr 98.1262
0.18 %
UET-13TR-EWS/ERG 72hr 10/02/2006 14:45:11 GSM214927 Probe variance for UET-13TR-EWS/ERG 72hr Artifact mask for UET-13TR-EWS/ERG 72hr 97.1368
0.17 %
UET-13TR-EWS/ERG 24hr tet+ 10/10/2006 14:00:18 GSM214928 Probe variance for UET-13TR-EWS/ERG 24hr tet+ Artifact mask for UET-13TR-EWS/ERG 24hr tet+ 97.8732
0.01 %
UET-13TR-EWS/ERG 48hr tet+ 10/10/2006 14:14:48 GSM214929 Probe variance for UET-13TR-EWS/ERG 48hr tet+ Artifact mask for UET-13TR-EWS/ERG 48hr tet+ 97.6718
0.31 %
UET-13TR-EWS/ERG72hr tet+ 10/02/2006 15:00:20 GSM214930 Probe variance for UET-13TR-EWS/ERG72hr tet+ Artifact mask for UET-13TR-EWS/ERG72hr tet+ 97.5859
0.07 %
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