Resting and IL2 activated human natural killer cells

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PubMed ID: none
Contact: Javeed Iqbal

Image:email GSE8059.png
University of Nebraska Medical Center

Species: Homo sapiens
Platform: Affymetrix GeneChip Human Genome U133 Plus 2.0 Array
Number of Samples: 6
Date Range: 08/12/2005 - 10/27/2005
Experiment Quality: 95.57
1.77 %
Link to Repository: GSE8059
Original Intensity Data: Download
Clean Intensity Data: Download
Original PLIER Data: Download
Clean PLIER Data: Download
Clean CEL Files:
Data Rank:

The aim of the study was to investigate the activation of human NK cells by IL2 through analyzing the global gene expression at different time points (0, 2, 8 and 24 hours) after culture with the cytokine IL2 at 100 IU/ml. NK cells with the CD56+/CD16+ and CD3- phenotype were negatively selected by immunomagnetic beads and re-examined by flow-cytometry to ensure greater than 90% purity .

Design: RNA was extracted and pooled from 4 different donors, amplified and labled according to the manufacturer’s instruction (GeneChipU133plus2® , Affymetrix Inc, CA).

Type: resting and IL2 activated NK cells(time series)


Contents

[edit] Contributors

  • Karen Dybkaer
  • Javeed Iqbal
  • Guimei Zhou
  • Huimin Geng
  • Li Xiao
  • Alexander Schmitz
  • Francesco d’Amore
  • Wing C Chan

[edit] Protocols

[edit] References

[edit] Links

GEO Accession for this experiment: GSE8059. (Public on Aug 07 2007)

RAW data: tar file.

[edit] Analysis

Gel Plots

Before Normalization

After Normalization & Cleaning

[edit] Samples/Quality Control

Sample Date Scanned Source Link Probe Variance Data Artifact Mask Quality Score
Resting_NK 08/12/2005 16:09:50 GSM198942 Probe variance for Resting_NK Artifact mask for Resting_NK 94.6771
0.06 %
NK_time2hours 08/12/2005 18:54:36 GSM198943 Probe variance for NK_time2hours Artifact mask for NK_time2hours 96.3633
0.29 %
NK_timepoint8hours 08/12/2005 16:41:55 GSM198944 Probe variance for NK_timepoint8hours Artifact mask for NK_timepoint8hours 96.7014
0.09 %
NK_timepoint24hours 08/12/2005 17:31:25 GSM198945 Probe variance for NK_timepoint24hours Artifact mask for NK_timepoint24hours 97.5706
0.04 %
Resting_CD8Tcells 10/27/2005 11:20:05 GSM198958 Probe variance for Resting_CD8Tcells Artifact mask for Resting_CD8Tcells 92.8881
8.23 %
Lymphoid control 10/13/2005 14:42:57 GSM198959 Probe variance for Lymphoid control Artifact mask for Lymphoid control 95.2354
1.90 %
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