Effect of EPO on erythroid maturation.

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PubMed ID: none
Contact: Jeffery Lynn Miller

Image:email_GSE7874.png
NIH/NIDDK

Species: Homo sapiens
Platform: Affymetrix GeneChip Human Genome U133 Plus 2.0 Array
Number of Samples: 10
Date Range: 06/14/2005 - 06/15/2005
Experiment Quality: 96.54
0.66 %
Link to Repository: GSE7874
Original Intensity Data: Download
Clean Intensity Data: Download
Original PLIER Data: Download
Clean PLIER Data: Download
Clean CEL Files:
Data Rank:

To study transcriptional profiles in human CD34+ cell in EPO condition on day 7 and day 14.

Design: After obtaining informed consent, human CD34+ cells were isolated in high purity from the peripheral blood of normal human volunteers. The cells were cultured at a concentration of 104 -105 cells/mL in medium supplemented with 4 U/mL EPO (Amgen, Thousand Oaks, CA) as described previously.1 Cells were enumerated using an electronic cell counter (Coulter, Hialeah, FL). Adult and fetal hemoglobin was analysed by HPLC. RNA was extracted using QIAshredder and Rneasy Minikit.

Type: time course


Contents

[edit] Contributors

  • Natarajan V Bhanu
  • Toshihiko Tanno
  • Terry Lee
  • Jeffery L Miller

[edit] Protocols

[edit] References

[edit] Links

GEO Accession for this experiment: GSE7874. (Public on Aug 22 2007)

RAW data: tar file.

[edit] Analysis

Gel Plots

Before Normalization

After Normalization & Cleaning

[edit] Samples/Quality Control

Sample Date Scanned Source Link Probe Variance Data Artifact Mask Quality Score
06-14-05_Miller_U133Plus2_0_A1P1d07EPO 06/15/05 13:03:52 GSM190964 Probe variance for 06-14-05_Miller_U133Plus2_0_A1P1d07EPO Artifact mask for 06-14-05_Miller_U133Plus2_0_A1P1d07EPO 94.4722
0 %
06-14-05_Miller_U133Plus2_0_A2P2d07EPO 06/14/05 16:04:25 GSM190965 Probe variance for 06-14-05_Miller_U133Plus2_0_A2P2d07EPO Artifact mask for 06-14-05_Miller_U133Plus2_0_A2P2d07EPO 97.0481
0.04 %
06-14-05_Miller_U133Plus2_0_A3P3d07EPO 06/14/05 14:22:52 GSM190966 Probe variance for 06-14-05_Miller_U133Plus2_0_A3P3d07EPO Artifact mask for 06-14-05_Miller_U133Plus2_0_A3P3d07EPO 96.7916
0.27 %
06-14-05_Miller_U133Plus2_0_A4P4d07EPO 06/14/05 16:35:28 GSM190967 Probe variance for 06-14-05_Miller_U133Plus2_0_A4P4d07EPO Artifact mask for 06-14-05_Miller_U133Plus2_0_A4P4d07EPO 96.928
0.09 %
06-14-05_Miller_U133Plus2_0_A5P5d07EPO 06/15/05 13:31:51 GSM190968 Probe variance for 06-14-05_Miller_U133Plus2_0_A5P5d07EPO Artifact mask for 06-14-05_Miller_U133Plus2_0_A5P5d07EPO 96.811
0.01 %
06-14-05_Miller_U133Plus2_0_A16P1d14EPO 06/15/05 13:16:52 GSM190969 Probe variance for 06-14-05_Miller_U133Plus2_0_A16P1d14EPO Artifact mask for 06-14-05_Miller_U133Plus2_0_A16P1d14EPO 95.5362
2.03 %
06-14-05_Miller_U133Plus2_0_A17P2d14EPO 06/14/05 15:51:24 GSM190970 Probe variance for 06-14-05_Miller_U133Plus2_0_A17P2d14EPO Artifact mask for 06-14-05_Miller_U133Plus2_0_A17P2d14EPO 97.4759
0.01 %
06-14-05_Miller_U133Plus2_0_A18P3d14EPO 06/14/05 13:57:00 GSM190971 Probe variance for 06-14-05_Miller_U133Plus2_0_A18P3d14EPO Artifact mask for 06-14-05_Miller_U133Plus2_0_A18P3d14EPO 96.7116
0.03 %
06-14-05_Miller_U133Plus2_0_A19P4d14EPO 06/14/05 16:22:38 GSM190972 Probe variance for 06-14-05_Miller_U133Plus2_0_A19P4d14EPO Artifact mask for 06-14-05_Miller_U133Plus2_0_A19P4d14EPO 96.9566
0.02 %
06-14-05_Miller_U133Plus2_0_A20P5d14EPO 06/15/05 13:44:44 GSM190973 Probe variance for 06-14-05_Miller_U133Plus2_0_A20P5d14EPO Artifact mask for 06-14-05_Miller_U133Plus2_0_A20P5d14EPO 96.6853
4.07 %
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