Transcriptional profiling of rhesus monkey nuclear transfer embryonic stem cells
From ArrayWiki
| PubMed ID: | none |
|---|---|
| Contact: | James Anthony Byrne
|
| Species: | Macaca mulatta |
| Platform: | Affymetrix GeneChip Rhesus Macaque Genome Array |
| Number of Samples: | 15 |
| Date Range: | 04/20/2007 - 04/20/2007 |
| Experiment Quality: | 95.14 1.08 % |
| Link to Repository: | GSE7748 |
| Original Intensity Data: | Download |
| Clean Intensity Data: | Download |
| Original PLIER Data: | Download |
| Clean PLIER Data: | Download |
| Clean CEL Files: | |
| Data Rank: | 
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Derivation of embryonic stem cells (ESC) genetically identical to a patient by somatic cell nuclear transfer (SCNT) holds the potential to cure or alleviate the symptoms of many degenerative diseases while circumventing any immunorejection issues. However, no primate nuclear transfer embryonic stem (ntES) cell lines have been derived to date. Here, we used a modified SCNT technique to produce rhesus macaque SCNT blastocysts at a relatively high efficiency from adult donor cells and we successfully derived two primate ntES cell lines from 304 oocytes (an overall efficiency of 0.7%). Nuclear and mitochondrial DNA analysis confirmed the ntES cell lines were derived from rhesus monkey SCNT blastocysts and both rhesus monkey ntES cell lines exhibited a normal ESC morphology, expressed key stemness markers, were transcriptionally indistinguishable from control ESC lines and differentiated into multiple cell types. This is, to our knowledge, the first confirmed derivation of primate ntES cell lines.
Design: We compared the transcriptomes of rhesus monkey embryonic stem cell lines derived from IVF-produced embryos (ORMES-10 and ORMES-22) with rhesus monkey nuclear transfer embryonic stem cells derived from somatic cell nuclear transfer produced embryos (CRES-1 and CRES-2). We also analyzed the donor adult somatic skin fibroblast cell line used to produce the cloned embryos. Three biological replicates of each cell line (A, B and C) was analyzed.
Type: rhesus monkey somatic cell nuclear transfer embryonic stem cells
Contents |
[edit] Contributors
- James A Byrne
- Darlene Perdersen
- Lisa Clepper
- Don Wolf
- Shoukhrat Mitalipov
[edit] Protocols
[edit] References
[edit] Links
GEO Accession for this experiment: GSE7748. (Public on Aug 29 2007)
RAW data: [Array tar file].
[edit] Analysis
 Before Normalization |
 After Normalization & Cleaning |
[edit] Samples/Quality Control
| Sample | Date Scanned | Source Link | Probe Variance Data | Artifact Mask | Quality Score |
|---|---|---|---|---|---|
| Somatic_cell_line_19822_ReplicateA | 04/20/07 15:35:49 | GSM187389 | 
| 
| 88.7625 4.21 % |
| Somatic_cell_line_19822_ReplicateB | 04/20/07 14:51:15 | GSM187390 | 
| 
| 90.5469 1.8 % |
| Somatic_cell_line_19822_ReplicateC | 04/20/07 13:00:18 | GSM187391 | 
| 
| 92.6564 3.31 % |
| ORMES10_ReplicateA | 04/20/07 11:53:03 | GSM187392 | 
| 
| 94.0425 0.03 % |
| ORMES10_ReplicateB | 04/20/07 12:26:50 | GSM187393 | 
| 
| 95.1372 0.01 % |
| ORMES10_ReplicateC | 04/20/07 15:13:33 | GSM187394 | 
| 
| 97.6673 0.03 % |
| ORMES22_ReplicateA | 04/20/07 12:15:36 | GSM187395 | 
| 
| 96.2098 0.06 % |
| ORMES22_ReplicateB | 04/20/07 12:37:51 | GSM187396 | 
| 
| 96.8135 5.05 % |
| ORMES22_ReplicateC | 04/20/07 12:04:19 | GSM187397 | 
| 
| 96.391 0.11 % |
| CRES1_ReplicateA | 04/20/07 12:49:04 | GSM187398 | 
| 
| 95.529 0.06 % |
| CRES1_ReplicateB | 04/20/07 15:24:34 | GSM187399 | 
| 
| 95.6501 0.01 % |
| CRES1_ReplicateC | 04/20/07 14:40:09 | GSM187400 | 
| 
| 96.3522 0.01 % |
| CRES2_ReplicateA | 04/20/07 15:47:01 | GSM187401 | 
| 
| 97.4646 0.02 % |
| CRES2_ReplicateB | 04/20/07 15:58:23 | GSM187402 | 
| 
| 96.4925 0.06 % |
| CRES2_ReplicateC | 04/20/07 15:02:28 | GSM187403 | 
| 
| 97.4053 1.36 % |
