Gene Expression and Biological Processes Influenced By Deletion of Stat3 in pulmonary Alveolar Type II Cells
From ArrayWiki
| PubMed ID: | none |
|---|---|
| Contact: | Yan Xu
|
| Species: | Mus musculus |
| Platform: | Affymetrix GeneChip Mouse Genome 430 2.0 Array |
| Number of Samples: | 6 |
| Date Range: | 06/22/2005 - 06/22/2005 |
| Experiment Quality: | 97.78 1.46 % |
| Link to Repository: | GSE6846 |
| Original Intensity Data: | Download |
| Clean Intensity Data: | Download |
| Original PLIER Data: | Download |
| Clean PLIER Data: | Download |
| Clean CEL Files: | |
| Data Rank: | 
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Deletion of Stat3 induced genes influencing protein metabolism, transport, chemotaxis and apoptosis and decreased the expression of genes mediating lipid synthesis and metabolism. Srebf1 and 2, key regulators of fatty acid and steroid biosynthesis, were decreased in Stat3D/D mice. Stat3 influenced both pro- and anti-apoptotic pathways, regulating and maintaining the balance between a subset of pro- and anti-apoptotic genes that determine cell death or survival. Akt, a known target of Stat3, participates in many Stat3 mediated pathways including Jak-Stat signaling, apoptosis, the MAPK signaling, cholesterol and fatty acid biosynthesis. Deletion of Stat3 from type II epithelial cells altered the expression of genes regulating diverse cellular processes, including cell growth and apoptosis, lipid biosynthesis and metabolism. Stat3 regulates cell formation through a complex regulatory network that likely enhances alveolar epithelial cell survival and surfactant/lipid synthesis, necessary for the protection of the lung during injury.
Design: To better understand the roles and molecular mechanisms by which Stat3 influences gene expression in lung, the effect of cell-selective deletion of Stat3 (Stat3D/D) on genome wide mRNA expression profiles was determined in murine type II alveolar epithelial cells. Differentially expressed genes were identified from Affymetrix Murine GeneChips analysis and subjected to gene ontology classification promoter analysis, pathway mapping and literature mining.
Type: genotype comparison
Contents |
[edit] Contributors
- Yan Xu
- Machiko Ikegami
- Yanhua Wang
- Yohei Matsuzaki
- Jeffrey A Whitsett
[edit] Protocols
[edit] References
[edit] Links
GEO Accession for this experiment: GSE6846. (Public on Aug 24 2007)
RAW data: tar file.
[edit] Analysis
 Before Normalization |
 After Normalization & Cleaning |
[edit] Samples/Quality Control
| Sample | Date Scanned | Source Link | Probe Variance Data | Artifact Mask | Quality Score |
|---|---|---|---|---|---|
| Knockout CS_2_9_KO_1 | 06/22/05 13:25:06 | GSM157806 | 
| 
| 97.0954 3.54 % |
| Knockout CS_2_15_KO_2 | 06/22/05 13:04:33 | GSM157807 | 
| 
| 97.9074 1.01 % |
| Knockout CS_MA_5_11_KO_3 | 06/22/05 13:56:09 | GSM157834 | 
| 
| 98.1428 0.27 % |
| Wild-type CS_12_29_1_WT_1 | 06/22/05 13:35:27 | GSM157835 | 
| 
| 97.613 0.29 % |
| Wild-type CS_12_29_2_WT_2 | 06/22/05 13:45:47 | GSM157836 | 
| 
| 98.1304 3.44 % |
| Wild-type CS_2_16_WT_3 | 06/22/05 13:14:40 | GSM157837 | 
| 
| 97.7686 0.23 % |
