Connectivity Map Batch 02 Random Load - CMAP5

From ArrayWiki

PubMed ID:	17008526
Contact:	Justin Lamb Broad Institute of MIT and Harvard
Species:	homo sapiens (cell lines)
Platform:	HT_HG-U133A
Number of Samples:	201
Date Range:	12/31/1969 - 12/31/1969
Experiment Quality:	93.91 0.94 % 96.49
Link to Repository:
Original Intensity Data:	Download
Clean Intensity Data:	Download
Original PLIER Data:	Download
Clean PLIER Data:	Download
Clean CEL Files:	Download
Data Rank:

To pursue a systematic approach to the discovery of functional connections among diseases, genetic perturbation, and drug action, we have created the first installment of a reference collection of gene-expression profiles from cultured human cells treated with bioactive small molecules, together with pattern-matching software to mine these data. We demonstrate that this 'Connectivity Map' resource can be used to find connections among small molecules sharing a mechanism of action, chemicals and physiological processes, and diseases and drugs. These results indicate the feasibility of the approach and suggest the value of a large-scale community Connectivity Map project.

Contents 1 Contributors 2 Protocols 3 References 4 Links 5 Analysis 6 Samples/Quality Control

[edit] Contributors

[edit] Protocols

[edit] References

[edit] Links

[edit] Analysis

Gel Plots

Before Normalization

After Normalization & Cleaning

[edit] Samples/Quality Control

Sample	Date Scanned	Source Link	Probe Variance Data	Artifact Mask	Quality Score
MCF7 perturbed by 1.3e-05microM of tolnaftate					94.0322 0 % 97.02
MCF7 perturbed by 1.38e-05microM of guanabenz					93.9148 0.21 % 96.85
MCF7 perturbed by 1.22e-05microM of niclosamide					94.0086 0 % 97
MCF7 perturbed by 1.18e-05microM of famotidine					93.408 0.94 % 96.23
MCF7 perturbed by 1.72e-05microM of metanephrine					93.5063 0 % 96.75
HL60 perturbed by 2.52e-05microM of betonicine					91.41 0.02 % 95.69
HL60 perturbed by 1.22e-05microM of pramocaine					91.9382 0.03 % 95.96
HL60 perturbed by 1.4e-05microM of harmaline					91.395 0.1 % 95.65
HL60 perturbed by 9.2e-06microM of fludroxycortide					91.1422 0.05 % 95.55
HL60 perturbed by 1.22e-05microM of dyclonine					92.2832 0.45 % 95.92
HL60 perturbed by 9.8e-06microM of glafenine					92.3964 0.6 % 95.9
HL60 perturbed by 1.36e-05microM of mebendazole					91.9795 0.41 % 95.79
vehicle					85.653 7.47 % 89.09
vehicle					88.7331 2.2 % 93.26
MCF7 perturbed by 0.0005microM of valproic acid					93.1579 0 % 96.58
MCF7 perturbed by 1.34e-05microM of noretynodrel					92.4733 0.05 % 96.21
MCF7 perturbed by 1.2e-05microM of hydroflumethiazide					92.4337 0.55 % 95.94
vehicle					92.7857 0.05 % 96.37
vehicle					89.6903 2.34 % 93.67
HL60 perturbed by 1.06e-05microM of droperidol					90.3389 1.58 % 94.38
HL60 perturbed by 1e-05microM of lumicolchicine					90.7931 0.04 % 95.38
HL60 perturbed by 5e-06microM of metrizamide					90.5811 0.71 % 94.94
PC3 perturbed by 1.66e-05microM of mefenamic acid					89.669 3.7 % 92.99
HL60 perturbed by 1.26e-05microM of imipramine					86.5995 0.19 % 93.2
PC3 perturbed by 1.46e-05microM of procaine					88.1831 0.06 % 94.06
vehicle					83.421 5.13 % 89.14
HL60 perturbed by 2.52e-05microM of allantoin					89.9153 1.32 % 94.3
HL60 perturbed by 4.4e-06microM of nystatin					91.1984 0.06 % 95.57
HL60 perturbed by 1.54e-05microM of tiaprofenic acid					90.5033 0.08 % 95.21
HL60 perturbed by 1.38e-05microM of epiandrosterone					90.3208 0.21 % 95.05
MCF7 perturbed by 1.26e-05microM of mefexamide					92.6517 0.67 % 95.99
HL60 perturbed by 1.06e-05microM of lithocholic acid					91.586 0.06 % 95.76
HL60 perturbed by 1.02e-05microM of Prestwick-689					91.9223 0.05 % 95.93
HL60 perturbed by 9.6e-06microM of cefalotin					91.7539 9.95 % 90.9
HL60 perturbed by 2.46e-05microM of anabasine					92.0174 0.19 % 95.91
MCF7 perturbed by 5e-06microM of iopromide					91.3321 0.46 % 95.44
MCF7 perturbed by 3.5e-05microM of thiamazole					89.2798 0.34 % 94.47
MCF7 perturbed by 1.08e-05microM of loracarbef					90.7371 4.09 % 93.33
MCF7 perturbed by 6.6e-06microM of lymecycline					89.6962 5.59 % 92.05
MCF7 perturbed by 1.08e-05microM of rimexolone					90.1055 5.36 % 92.37
MCF7 perturbed by 1.22e-05microM of cyclopentolate					89.5079 4.59 % 92.46
MCF7 perturbed by 1.2e-05microM of piribedil					89.8083 4.76 % 92.52
HL60 perturbed by 0.0005microM of valproic acid					90.4255 13.56 % 88.43
HL60 perturbed by 9.2e-06microM of clidinium bromide					90.6791 5.71 % 92.49
HL60 perturbed by 1e-06microM of tanespimycin					90.5025 0.05 % 95.23
MCF7 perturbed by 7.2e-06microM of cefotiam					93.446 0.05 % 96.7
MCF7 perturbed by 6.8e-06microM of doxorubicin					90.5442 0.1 % 95.22
MCF7 perturbed by 4.6e-06microM of paclitaxel					92.3884 4.72 % 93.83
MCF7 perturbed by 1.4e-05microM of lynestrenol					92.4217 7.99 % 92.21
MCF7 perturbed by 2.22e-05microM of caffeic acid					91.6583 8.48 % 91.59
MCF7 perturbed by 2.24e-05microM of propofol					93.1897 5.82 % 93.68
PC3 perturbed by 9.4e-06microM of fludrocortisone					88.7733 5.89 % 91.44
PC3 perturbed by 1e-07microM of trichostatin A					90.5731 1.43 % 94.57
PC3 perturbed by 7.8e-06microM of eldeline					88.7065 3.02 % 92.84
MCF7 perturbed by 1e-06microM of PNU-0230031					89.9111 1.83 % 94.04
vehicle					89.8373 0.85 % 94.49
MCF7 perturbed by 1.08e-05microM of (+)-chelidonine					89.0908 14.9 % 87.1
MCF7 perturbed by 1.04e-05microM of racecadotril					89.7111 0.1 % 94.81
MCF7 perturbed by 1.02e-05microM of alpha-yohimbine					92.3522 0 % 96.18
MCF7 perturbed by 5.6e-06microM of demecarium bromide					92.581 0.01 % 96.28
MCF7 perturbed by 2.1e-05microM of pseudopelletierine					91.1412 3.17 % 93.99
MCF7 perturbed by 1.08e-05microM of bicuculline					92.6014 0.09 % 96.25
MCF7 perturbed by 1.42e-05microM of guanfacine					93.0094 3.3 % 94.85
MCF7 perturbed by 1e-07microM of trichostatin A					93.1299 0.26 % 96.44
MCF7 perturbed by 1.22e-05microM of prasterone					93.1655 0.72 % 96.22
MCF7 perturbed by 8.4e-06microM of fluspirilene					92.6699 4.01 % 94.33
vehicle					93.4625 0.14 % 96.66
vehicle					91.9952 2.66 % 94.67
MCF7 perturbed by 1.88e-05microM of felbinac					93.1066 0 % 96.55
MCF7 perturbed by 1.4e-05microM of luteolin					93.2781 0 % 96.64
MCF7 perturbed by 2.22e-05microM of theobromine					93.3973 0 % 96.7
MCF7 perturbed by 9.4e-06microM of trimethobenzamide					93.0396 0.48 % 96.28
vehicle					92.1575 0.83 % 95.66
MCF7 perturbed by 9.4e-06microM of fludrocortisone					92.4439 2.51 % 94.97
MCF7 perturbed by 7.8e-06microM of eldeline					92.219 1.42 % 95.4
vehicle					91.1587 0 % 95.58
PC3 perturbed by 1e-05microM of 0175029-0000					90.7742 3.6 % 93.59
PC3 perturbed by 1.04e-05microM of lomefloxacin					91.8327 0.01 % 95.91
vehicle					91.7151 1.26 % 95.23
HL60 perturbed by 8.8e-06microM of terguride					91.7786 0.16 % 95.81
HL60 perturbed by 9e-06microM of bephenium hydroxynaphthoate					91.967 0.03 % 95.97
HL60 perturbed by 2.04e-05microM of meglumine					91.7955 0.03 % 95.88
MCF7 perturbed by 1.58e-05microM of harmol					92.6842 0.5 % 96.09
MCF7 perturbed by 5.2e-06microM of tubocurarine chloride					92.1454 0.8 % 95.67
MCF7 perturbed by 1.04e-05microM of benperidol					90.4566 1.78 % 94.34
PC3 perturbed by 1e-05microM of CP-320650-01					92.217 0.6 % 95.81
PC3 perturbed by 1.24e-05microM of lycorine					91.4703 1.37 % 95.05
PC3 perturbed by 1.22e-05microM of pramocaine					92.2572 0.79 % 95.73
PC3 perturbed by 1.48e-05microM of tolbutamide					92.6177 0.43 % 96.09
PC3 perturbed by 1e-07microM of trichostatin A					92.1676 0.78 % 95.69
PC3 perturbed by 1.18e-05microM of canadine					90.0704 2.21 % 93.93
MCF7 perturbed by 2.8e-06microM of streptomycin					93.1662 3.99 % 94.59
vehicle					93.7708 2.15 % 95.81
PC3 perturbed by 4.8e-06microM of mebhydrolin					91.9895 0.59 % 95.7
PC3 perturbed by 2.58e-05microM of Prestwick-967					91.0369 1.21 % 94.91
PC3 perturbed by 1.54e-05microM of norcyclobenzaprine					91.6864 1.27 % 95.21
PC3 perturbed by 1.64e-05microM of salsolidin					91.6056 1.04 % 95.28
HL60 perturbed by 7.4e-06microM of doxazosin					91.8837 3.1 % 94.39
MCF7 perturbed by 1.84e-05microM of ethoxyquin					92.1714 0.9 % 95.64
MCF7 perturbed by 1.48e-05microM of tolbutamide					92.3494 3.2 % 94.57
MCF7 perturbed by 7.8e-06microM of eldeline					92.8989 0.64 % 96.13
HL60 perturbed by 1.3e-05microM of sotalol					90.8625 4.18 % 93.34
MCF7 perturbed by 1.18e-05microM of quercetin					92.3485 1.84 % 95.26
MCF7 perturbed by 1.12e-05microM of succinylsulfathiazole					91.7334 2.63 % 94.55
MCF7 perturbed by 1.28e-05microM of cyclobenzaprine					92.9352 1.83 % 95.55
MCF7 perturbed by 1.42e-05microM of indoprofen					92.6338 4.39 % 94.12
MCF7 perturbed by 1.12e-05microM of conessine					92.4211 0.8 % 95.81
vehicle					90.6769 0.56 % 95.06
PC3 perturbed by 1.06e-05microM of lobelanidine					90.6756 2.04 % 94.32
PC3 perturbed by 1.6e-05microM of sulfadiazine					88.9623 4.73 % 92.12
PC3 perturbed by 1.34e-05microM of norethisterone					91.2143 1.73 % 94.74
MCF7 perturbed by 7.4e-06microM of Prestwick-692					93.3336 0.35 % 96.49
MCF7 perturbed by 1.96e-05microM of 3-acetamidocoumarin					93.2766 0 % 96.64
MCF7 perturbed by 3.4e-06microM of ciclosporin					93.4024 0.11 % 96.65
MCF7 perturbed by 1.72e-05microM of captopril					92.4701 0 % 96.24
PC3 perturbed by 1.78e-05microM of aciclovir					92.7417 0.29 % 96.23
MCF7 perturbed by 2.2e-05microM of mephenesin					91.5247 1.07 % 95.23
vehicle					92.7551 0.51 % 96.12
PC3 perturbed by 1e-06microM of geldanamycin					92.2035 0.64 % 95.78
PC3 perturbed by 1.04e-05microM of altizide					93.226 1.09 % 96.07
PC3 perturbed by 1.72e-05microM of aminophenazone					92.6909 0.51 % 96.09
vehicle					92.626 0.12 % 96.25
PC3 perturbed by 1.28e-05microM of maprotiline					92.9734 1.51 % 95.73
PC3 perturbed by 2.58e-05microM of bemegride					92.7958 0.04 % 96.38
PC3 perturbed by 1.04e-05microM of brinzolamide					92.9123 0.01 % 96.45
PC3 perturbed by 1e-05microM of hexamethonium bromide					92.73 0.21 % 96.26
PC3 perturbed by 1.48e-05microM of estrone					92.9527 0 % 96.47
PC3 perturbed by 1.36e-05microM of propranolol					92.7276 0.71 % 96.01
PC3 perturbed by 1.24e-05microM of cyproheptadine					91.3282 3.45 % 93.94
PC3 perturbed by 1.42e-05microM of N6-methyladenosine					92.0581 0.96 % 95.55
MCF7 perturbed by 1.56e-05microM of procarbazine					93.7176 0.51 % 96.6
vehicle					94.1047 0.1 % 97
MCF7 perturbed by 1.26e-05microM of imipramine					94.1611 0.41 % 96.88
MCF7 perturbed by 3.4e-06microM of pyrvinium					93.3029 0 % 96.65
MCF7 perturbed by 1.14e-05microM of ranitidine					93.4158 0.22 % 96.6
PC3 perturbed by 2.6e-06microM of gentamicin					91.5665 0.29 % 95.64
MCF7 perturbed by 8.2e-06microM of isometheptene					92.3532 0 % 96.18
MCF7 perturbed by 1.12e-05microM of indometacin					92.7592 0.01 % 96.38
MCF7 perturbed by 8.6e-06microM of pivampicillin					92.072 0.03 % 96.02
PC3 perturbed by 1.08e-05microM of (+)-chelidonine					90.9726 0 % 95.49
PC3 perturbed by 7.4e-06microM of cefepime					93.0454 0.01 % 96.52
PC3 perturbed by 9.4e-06microM of fipexide					93.2405 0.39 % 96.43
PC3 perturbed by 1.62e-05microM of ellipticine					92.1547 0 % 96.08
MCF7 perturbed by 4.8e-06microM of josamycin					92.4123 2.5 % 94.95
MCF7 perturbed by 1.76e-05microM of metyrapone					91.3522 1.34 % 95.01
MCF7 perturbed by 1e-08microM of estradiol					93.4591 1 % 96.23
PC3 perturbed by 1e-05microM of PF-00875133-00					92.9612 0 % 96.48
vehicle					92.5385 0.12 % 96.21
MCF7 perturbed by 1e-05microM of pioglitazone					92.6563 1.61 % 95.52
MCF7 perturbed by 9.2e-06microM of timolol					92.711 0.91 % 95.9
MCF7 perturbed by 1.5e-05microM of equilin					90.8216 0.01 % 95.41
MCF7 perturbed by 1.12e-05microM of epivincamine					93.0345 2.1 % 95.47
MCF7 perturbed by 9e-06microM of acepromazine					92.5843 2.18 % 95.2
MCF7 perturbed by 8.4e-06microM of isopropamide iodide					92.474 0.11 % 96.18
MCF7 perturbed by 1.12e-05microM of napelline					93.0534 0.09 % 96.48
MCF7 perturbed by 1.04e-05microM of dipivefrine					91.9351 2.13 % 94.9
MCF7 perturbed by 1.3e-05microM of nifenazone					93.3001 0.01 % 96.64
MCF7 perturbed by 1.08e-05microM of (+)-chelidonine					93.0771 1.67 % 95.7
MCF7 perturbed by 1.32e-05microM of mianserin					93.7671 0.06 % 96.85
vehicle					93.6081 1.1 % 96.25
MCF7 perturbed by 9.6e-06microM of naltrexone					93.6777 0.58 % 96.55
HL60 perturbed by 1e-05microM of thioridazine					90.047 3.4 % 93.32
HL60 perturbed by 1e-07microM of alvespimycin					89.7163 4.51 % 92.6
HL60 perturbed by 1e-04microM of acetylsalicylic acid					90.7066 4.18 % 93.26
PC3 perturbed by 9.6e-06microM of prazosin					91.2355 2.9 % 94.17
PC3 perturbed by 6.6e-06microM of benzonatate					91.0558 3.73 % 93.66
PC3 perturbed by 1.56e-05microM of ganciclovir					91.194 3.37 % 93.91
PC3 perturbed by 1.2e-05microM of proxymetacaine					90.5968 3.96 % 93.32
vehicle					77.427 1.46 % 87.98
vehicle					93.1499 0.42 % 96.36
PC3 perturbed by 1e-05microM of valdecoxib					93.2304 0.7 % 96.26
PC3 perturbed by 1e-05microM of STOCK1N-35215					93.0539 0.4 % 96.33
MCF7 perturbed by 6e-06microM of nadide					93.9018 0 % 96.95
MCF7 perturbed by 1.12e-05microM of napelline					93.1361 0 % 96.57
MCF7 perturbed by 1.76e-05microM of trioxysalen					93.9622 0 % 96.98
vehicle					91.485 0.01 % 95.74
vehicle					83.6041 1.02 % 91.29
MCF7 perturbed by 1e-07microM of trichostatin A					80.038 0.16 % 89.94
vehicle					91.2069 0.13 % 95.54
PC3 perturbed by 1e-05microM of 5155877					92.3824 0.31 % 96.03
MCF7 perturbed by 1e-05microM of 5155877					92.5624 0.55 % 96.01
PC3 perturbed by 1e-05microM of 16-16-dimethylprostaglandin E2					90.7028 3.74 % 93.48
vehicle					92.5423 0.13 % 96.21
MCF7 perturbed by 5e-05microM of valproic acid					83.3835 0.55 % 91.42
MCF7 perturbed by 0.001microM of valproic acid					81.6904 2.23 % 89.73
MCF7 perturbed by 1e-05microM of bambuterol					84.0645 0.82 % 91.62
MCF7 perturbed by 2.62e-05microM of mesalazine					83.6613 0.36 % 91.65
vehicle					83.0137 8.55 % 87.23
MCF7 perturbed by 8.8e-06microM of ursolic acid					85.555 0.01 % 92.77
MCF7 perturbed by 8.6e-06microM of delsoline					80.524 2.63 % 88.95
PC3 perturbed by 8.2e-06microM of enalapril					83.3454 2.72 % 90.31
PC3 perturbed by 9.8e-06microM of glafenine					82.54 1.58 % 90.48
PC3 perturbed by 1.1e-05microM of atropine methonitrate					80.3081 1.54 % 89.38
PC3 perturbed by 8.8e-06microM of levocabastine					81.1433 0.78 % 90.18
vehicle					77.8825 1.29 % 88.29
PC3 perturbed by 1e-06microM of 0175029-0000					76.2423 0.28 % 87.98
MCF7 perturbed by 1e-05microM of pioglitazone					75.7545 1.19 % 87.28
PC3 perturbed by 5e-07microM of colforsin					78.7023 0.01 % 89.35
vehicle					77.454 0.62 % 88.42
MCF7 perturbed by 1e-07microM of trichostatin A					78.473 0.05 % 89.21
PC3 perturbed by 1e-05microM of 11-deoxy-16-16-dimethylprostaglandin E2					81.36 2.81 % 89.27