Connectivity Map Batch 02 Random Load - CMAP31

From ArrayWiki

PubMed ID:	17008526
Contact:	Justin Lamb Broad Institute of MIT and Harvard
Species:	homo sapiens (cell lines)
Platform:	HT_HG-U133A
Number of Samples:	201
Date Range:	12/31/1969 - 12/31/1969
Experiment Quality:	87.99 2.29 % 92.85
Link to Repository:
Original Intensity Data:	Download
Clean Intensity Data:	Download
Original PLIER Data:	Download
Clean PLIER Data:	Download
Clean CEL Files:	Download
Data Rank:

To pursue a systematic approach to the discovery of functional connections among diseases, genetic perturbation, and drug action, we have created the first installment of a reference collection of gene-expression profiles from cultured human cells treated with bioactive small molecules, together with pattern-matching software to mine these data. We demonstrate that this 'Connectivity Map' resource can be used to find connections among small molecules sharing a mechanism of action, chemicals and physiological processes, and diseases and drugs. These results indicate the feasibility of the approach and suggest the value of a large-scale community Connectivity Map project.

Contents 1 Contributors 2 Protocols 3 References 4 Links 5 Analysis 6 Samples/Quality Control

[edit] Contributors

[edit] Protocols

[edit] References

[edit] Links

[edit] Analysis

Gel Plots

Before Normalization

After Normalization & Cleaning

[edit] Samples/Quality Control

Sample	Date Scanned	Source Link	Probe Variance Data	Artifact Mask	Quality Score
HL60 perturbed by 1e-05microM of LY-294002					88.5037 0.92 % 93.79
PC3 perturbed by 1e-05microM of clozapine					87.9877 0.44 % 93.77
PC3 perturbed by 1e-06microM of tanespimycin					87.0006 0.13 % 93.44
HL60 perturbed by 0.0002microM of valproic acid					86.7521 2.29 % 92.23
MCF7 perturbed by 1.78e-05microM of aciclovir					92.0831 0.28 % 95.9
MCF7 perturbed by 1.48e-05microM of lidocaine					93.6811 0.04 % 96.82
MCF7 perturbed by 1.02e-05microM of diphemanil metilsulfate					92.4422 0.12 % 96.16
vehicle					93.5783 0 % 96.79
MCF7 perturbed by 2.02e-05microM of levodopa					93.7141 0 % 96.86
MCF7 perturbed by 5.4e-06microM of erythromycin					93.298 0 % 96.65
MCF7 perturbed by 1.1e-05microM of clemizole					91.4762 0.37 % 95.55
HL60 perturbed by 1.18e-05microM of trihexyphenidyl					90.9196 0.15 % 95.39
HL60 perturbed by 4.6e-06microM of solanine					90.3036 3.67 % 93.32
HL60 perturbed by 1.4e-05microM of sulfachlorpyridazine					91.5846 0.03 % 95.78
HL60 perturbed by 7.2e-06microM of emetine					89.8933 0.04 % 94.93
vehicle					90.8606 0.06 % 95.4
HL60 perturbed by 1.28e-05microM of ethisterone					91.7537 3.89 % 93.93
HL60 perturbed by 1.64e-05microM of pinacidil					92.0159 0.47 % 95.77
HL60 perturbed by 7.4e-06microM of pentolonium					91.985 0.06 % 95.96
HL60 perturbed by 1.68e-05microM of salbutamol					91.7796 1.85 % 94.96
vehicle					91.9314 0.13 % 95.9
PC3 perturbed by 1.32e-05microM of mianserin					88.2067 1.72 % 93.24
PC3 perturbed by 9.6e-06microM of spironolactone					88.3026 2.94 % 92.68
PC3 perturbed by 8.6e-06microM of clindamycin					88.5862 1.68 % 93.45
MCF7 perturbed by 1.26e-05microM of imipramine					92.8188 0 % 96.41
MCF7 perturbed by 1e-07microM of LY-294002					93.3781 0.05 % 96.66
MCF7 perturbed by 2.52e-05microM of allantoin					92.991 0 % 96.5
HL60 perturbed by 1.38e-05microM of androsterone					89.5237 3.77 % 92.88
HL60 perturbed by 1.22e-05microM of carteolol					90.2217 0.4 % 94.91
HL60 perturbed by 7.6e-06microM of ketoconazole					89.7311 1.66 % 94.04
HL60 perturbed by 6.6e-06microM of oxantel					89.8667 1.89 % 93.99
HL60 perturbed by 9.2e-06microM of dl-alpha tocopherol					90.8541 0.06 % 95.4
vehicle					90.4232 2.32 % 94.05
PC3 perturbed by 1.12e-05microM of chlorpromazine					89.5472 0.82 % 94.36
HL60 perturbed by 1.08e-05microM of morantel					86.2903 2.1 % 92.09
HL60 perturbed by 2.62e-05microM of 8-azaguanine					86.9416 7.52 % 89.71
HL60 perturbed by 1.62e-05microM of methoxamine					89.7966 0.14 % 94.83
HL60 perturbed by 1.04e-05microM of colecalciferol					90.8279 0.14 % 95.34
MCF7 perturbed by 1.6e-05microM of gemfibrozil					92.5242 1.14 % 95.69
MCF7 perturbed by 1.12e-05microM of aceclofenac					93.0033 0.52 % 96.24
HL60 perturbed by 5.8e-06microM of monensin					89.9069 1.49 % 94.21
HL60 perturbed by 1.24e-05microM of sulfaquinoxaline					91.0152 0.06 % 95.48
vehicle					91.427 0.29 % 95.57
MCF7 perturbed by 9.6e-06microM of coralyne					92.6833 0.23 % 96.23
MCF7 perturbed by 9.4e-06microM of buspirone					93.0133 0.04 % 96.49
MCF7 perturbed by 8.4e-06microM of doxycycline					91.4449 0.02 % 95.71
MCF7 perturbed by 1.16e-05microM of bergenin					91.5112 0.07 % 95.72
vehicle					89.6711 0 % 94.83
MCF7 perturbed by 1.42e-05microM of sulfametoxydiazine					92.9151 0.9 % 96.01
MCF7 perturbed by 8e-06microM of demeclocycline					92.6519 0 % 96.33
MCF7 perturbed by 1.96e-05microM of Prestwick-1103					90.7898 4.32 % 93.23
MCF7 perturbed by 1.62e-05microM of (-)-isoprenaline					90.2455 4.12 % 93.06
MCF7 perturbed by 1.38e-05microM of estriol					90.0696 4.99 % 92.54
HL60 perturbed by 9.2e-06microM of pipenzolate bromide					89.9111 0.22 % 94.84
MCF7 perturbed by 1.16e-05microM of fluoxetine					91.0979 0 % 95.55
MCF7 perturbed by 1.86e-05microM of methoxsalen					92.9911 0.01 % 96.49
MCF7 perturbed by 1e-05microM of lumicolchicine					90.0537 0.09 % 94.98
vehicle					91.9759 7.67 % 92.15
MCF7 perturbed by 8.6e-06microM of cisapride					92.0973 5.09 % 93.5
MCF7 perturbed by 1.48e-05microM of pilocarpine					92.8398 2.79 % 95.02
MCF7 perturbed by 1.32e-05microM of neostigmine bromide					93.3111 0.53 % 96.39
PC3 perturbed by 1.12e-05microM of vincamine					91.393 0.59 % 95.4
PC3 perturbed by 1.1e-05microM of furaltadone					89.901 0.09 % 94.91
MCF7 perturbed by 1.3e-05microM of foliosidine					88.8892 0.33 % 94.28
MCF7 perturbed by 1e-05microM of PNU-0230031					88.4257 2.21 % 93.11
MCF7 perturbed by 1.58e-05microM of fenbufen					88.9866 0.95 % 94.02
MCF7 perturbed by 1.28e-05microM of levonorgestrel					88.0337 0.11 % 93.96
MCF7 perturbed by 4.6e-06microM of solanine					89.5524 13.08 % 88.23
MCF7 perturbed by 1.02e-05microM of yohimbine					90.9717 0 % 95.49
vehicle					92.1533 0.35 % 95.9
MCF7 perturbed by 1.02e-05microM of pentetic acid					92.3716 0.09 % 96.14
MCF7 perturbed by 1e-07microM of trichostatin A					91.6895 3.65 % 94.02
MCF7 perturbed by 7.4e-06microM of doxazosin					91.855 0.1 % 95.88
MCF7 perturbed by 1.02e-05microM of digoxigenin					90.51 0 % 95.26
MCF7 perturbed by 1.12e-05microM of chlorogenic acid					92.2651 1.2 % 95.54
MCF7 perturbed by 6.6e-06microM of carbenoxolone					92.6884 0.25 % 96.22
MCF7 perturbed by 1.4e-05microM of sulmazole					92.1764 0.8 % 95.69
MCF7 perturbed by 1.58e-05microM of cimetidine					92.2313 1.13 % 95.55
vehicle					91.4436 1.32 % 95.06
PC3 perturbed by 8.2e-06microM of cefamandole					90.2734 0 % 95.14
vehicle					89.2918 3.59 % 92.85
HL60 perturbed by 1.26e-05microM of nilutamide					91.1075 0.02 % 95.55
HL60 perturbed by 1.76e-05microM of metyrapone					91.1124 0.14 % 95.49
HL60 perturbed by 1.02e-05microM of ursodeoxycholic acid					91.1181 0.02 % 95.55
MCF7 perturbed by 1.22e-05microM of ajmaline					92.0436 0.2 % 95.92
MCF7 perturbed by 2.8e-06microM of sisomicin					92.1543 2.3 % 94.93
MCF7 perturbed by 9.6e-06microM of prenylamine					91.2617 1.57 % 94.84
MCF7 perturbed by 1.76e-05microM of resveratrol					91.9747 0.54 % 95.72
vehicle					92.6705 0.44 % 96.12
PC3 perturbed by 9.4e-06microM of fludrocortisone					91.7987 1.12 % 95.34
PC3 perturbed by 1e-05microM of CP-690334-01					91.5988 1.1 % 95.25
PC3 perturbed by 1e-06microM of withaferin A					92.2839 0.78 % 95.75
PC3 perturbed by 1e-05microM of 0317956-0000					92.3283 0.99 % 95.67
PC3 perturbed by 8.6e-06microM of methylbenzethonium chloride					92.0769 0.09 % 95.99
vehicle					92.2904 1.28 % 95.51
PC3 perturbed by 1.52e-05microM of homosalate					92.3342 1.86 % 95.24
PC3 perturbed by 1.24e-05microM of etifenin					91.2122 0.86 % 95.18
PC3 perturbed by 1.78e-05microM of selegiline					90.4853 1.66 % 94.41
MCF7 perturbed by 1.44e-05microM of bupropion					92.5981 6.08 % 93.26
MCF7 perturbed by 1.34e-05microM of guanethidine					93.1212 9.27 % 91.92
PC3 perturbed by 1.16e-05microM of buflomedil					90.5168 5.14 % 92.69
PC3 perturbed by 2.2e-05microM of carcinine					89.7554 0.98 % 94.39
HL60 perturbed by 9.8e-06microM of thioperamide					90.4413 0.33 % 95.05
HL60 perturbed by 2.1e-05microM of tetroquinone					91.5931 4.1 % 93.74
HL60 perturbed by 1.84e-05microM of fosfosal					91.0319 3.2 % 93.92
vehicle					91.3786 1.18 % 95.1
MCF7 perturbed by 9.4e-06microM of bendroflumethiazide					91.3884 0.41 % 95.49
MCF7 perturbed by 9e-06microM of Prestwick-1100					91.8257 1.04 % 95.39
HL60 perturbed by 1.52e-05microM of ozagrel					91.6449 0.15 % 95.75
HL60 perturbed by 8.6e-06microM of pivampicillin					91.248 3.49 % 93.88
vehicle					92.5256 2.32 % 95.1
PC3 perturbed by 8.2e-06microM of isometheptene					91.2895 1.81 % 94.74
PC3 perturbed by 7.6e-06microM of mometasone					90.3001 1.98 % 94.16
PC3 perturbed by 1.04e-05microM of imidurea					90.2356 2.97 % 93.63
PC3 perturbed by 1.58e-05microM of diprophylline					92.1715 1.82 % 95.17
PC3 perturbed by 6.2e-06microM of ceftazidime					88.4565 6.28 % 91.09
PC3 perturbed by 1.7e-05microM of carbamazepine					89.1807 2.34 % 93.42
PC3 perturbed by 1.5e-05microM of diethylstilbestrol					91.278 2.59 % 94.34
PC3 perturbed by 3.5e-05microM of thiamazole					90.9465 0.57 % 95.19
PC3 perturbed by 3.4e-06microM of ciclosporin					89.9771 3.23 % 93.38
vehicle					92.0759 0.7 % 95.69
PC3 perturbed by 1.6e-05microM of etidronic acid					92.3577 0.72 % 95.82
MCF7 perturbed by 1.82e-05microM of harman					93.2991 0.25 % 96.52
PC3 perturbed by 4.8e-06microM of josamycin					92.626 0.37 % 96.13
vehicle					92.3063 0.56 % 95.88
PC3 perturbed by 1.16e-05microM of betaxolol					91.4214 1.17 % 95.12
MCF7 perturbed by 1.36e-05microM of meclofenoxate					93.2812 1.58 % 95.85
PC3 perturbed by 1.22e-05microM of prasterone					92.5144 0.07 % 96.22
PC3 perturbed by 6.6e-06microM of prochlorperazine					92.624 0.06 % 96.28
PC3 perturbed by 1.1e-05microM of bezafibrate					92.3557 0 % 96.18
PC3 perturbed by 8.8e-06microM of diltiazem					90.8678 0.4 % 95.24
PC3 perturbed by 9e-06microM of loxapine					91.4373 1.33 % 95.06
PC3 perturbed by 2.04e-05microM of meglumine					91.5796 1.28 % 95.15
MCF7 perturbed by 1.86e-05microM of 10-methoxyharmalan					92.7737 0.14 % 96.32
MCF7 perturbed by 1e-07microM of trichostatin A					93.3341 0.25 % 96.54
MCF7 perturbed by 1.5e-05microM of azacyclonol					93.5912 0.17 % 96.71
PC3 perturbed by 4.2e-06microM of neomycin					89.7921 0.19 % 94.8
PC3 perturbed by 8.6e-06microM of delsoline					91.6065 0.58 % 95.51
MCF7 perturbed by 1.06e-05microM of lobelanidine			Image:5500024034290101707045.A02.CEL probe-variance.png		90.9735 0.11 % 95.43
MCF7 perturbed by 1.58e-05microM of chrysin					91.7071 0.24 % 95.74
PC3 perturbed by 1.04e-05microM of racecadotril					91.5182 0 % 95.76
vehicle					92.2584 1.68 % 95.29
PC3 perturbed by 1.46e-05microM of ebselen					92.198 0 % 96.1
MCF7 perturbed by 2.28e-05microM of hymecromone					90.9179 0.91 % 95
vehicle					89.6237 2.76 % 93.43
MCF7 perturbed by 9.6e-06microM of dehydrocholic acid					92.0233 3.48 % 94.27
MCF7 perturbed by 1.48e-05microM of procainamide					89.5032 1.26 % 94.12
PC3 perturbed by 1e-05microM of pioglitazone					92.2386 0.13 % 96.05
PC3 perturbed by 1e-05microM of LY-294002					91.5768 0.28 % 95.65
vehicle					92.1919 1.02 % 95.59
PC3 perturbed by 1e-05microM of pioglitazone					91.0103 2.39 % 94.31
MCF7 perturbed by 0.0005microM of valproic acid					89.3763 1.82 % 93.78
MCF7 perturbed by 1e-05microM of troglitazone					89.7233 0.79 % 94.47
MCF7 perturbed by 1.84e-05microM of securinine					92.1903 0.07 % 96.06
MCF7 perturbed by 1.32e-05microM of hydrocotarnine					91.9513 1.05 % 95.45
MCF7 perturbed by 1.06e-05microM of riboflavin					92.2352 1.71 % 95.26
MCF7 perturbed by 1.14e-05microM of drofenine					91.538 0 % 95.77
MCF7 perturbed by 8e-06microM of sertaconazole					91.9413 0 % 95.97
MCF7 perturbed by 8.6e-06microM of moracizine					92.4436 0 % 96.22
MCF7 perturbed by 1.2e-05microM of dosulepin					91.4649 1.22 % 95.12
MCF7 perturbed by 1.22e-05microM of ondansetron					93.5145 0.3 % 96.61
MCF7 perturbed by 5.2e-06microM of saquinavir					92.8956 3.13 % 94.88
MCF7 perturbed by 9.6e-06microM of mefloquine					93.2815 0.11 % 96.59
HL60 perturbed by 0.0005microM of valproic acid					87.3437 3.82 % 91.76
vehicle					90.2123 0.01 % 95.1
PC3 perturbed by 8.4e-06microM of alverine					90.6514 3.4 % 93.63
PC3 perturbed by 2.32e-05microM of pralidoxime					90.7773 1.6 % 94.59
PC3 perturbed by 8.2e-06microM of verapamil					91.1633 1.86 % 94.65
vehicle					78.303 1.94 % 88.18
MCF7 perturbed by 1.4e-05microM of acacetin					91.1176 0 % 95.56
MCF7 perturbed by 1.16e-05microM of bromopride					92.3353 0.01 % 96.16
MCF7 perturbed by 1.48e-05microM of hexestrol					92.8728 0 % 96.44
MCF7 perturbed by 1.96e-05microM of mecamylamine					82.6525 1.97 % 90.34
MCF7 perturbed by 1e-04microM of acetylsalicylic acid					84.9974 0.02 % 92.49
MCF7 perturbed by 1.22e-05microM of desoxycortone					91.5731 1.76 % 94.91
vehicle					92.3195 0.2 % 96.06
MCF7 perturbed by 1.3e-05microM of clioquinol					92.0062 0.16 % 95.92
PC3 perturbed by 1.4e-05microM of STOCK1N-35874					90.8033 3.65 % 93.57
MCF7 perturbed by 0.0002microM of valproic acid					83.8236 2.07 % 90.88
MCF7 perturbed by 1e-06microM of chlorpromazine					83.6299 2.77 % 90.43
MCF7 perturbed by 5.4e-06microM of alimemazine					84.0849 1.15 % 91.47
MCF7 perturbed by 9.4e-06microM of terazosin					86.4246 0.55 % 92.94
MCF7 perturbed by 1.22e-05microM of azaperone					83.35 0.99 % 91.18
vehicle					84.5408 3.03 % 90.76
MCF7 perturbed by 1.02e-05microM of nalbuphine					84.2992 0.77 % 91.77
MCF7 perturbed by 4.2e-06microM of neomycin					85.5198 0.36 % 92.58
MCF7 perturbed by 1.12e-05microM of nomifensine					85.1589 0.16 % 92.5
MCF7 perturbed by 9.6e-06microM of noscapine					84.8127 0.04 % 92.39
PC3 perturbed by 1.38e-05microM of diphenhydramine					83.4145 2.74 % 90.34
PC3 perturbed by 1.24e-05microM of naftifine					84.892 3.7 % 90.6
PC3 perturbed by 1.42e-05microM of artemisinin					82.9787 0.68 % 91.15
PC3 perturbed by 1.28e-05microM of sulfadimethoxine					79.1508 0.08 % 89.53
MCF7 perturbed by 1.5e-05microM of zardaverine					75.6041 0.22 % 87.69
PC3 perturbed by 1e-06microM of 0173570-0000					77.2595 1.21 % 88.03
MCF7 perturbed by 1e-07microM of MG-262					79.5877 0.93 % 89.33
PC3 perturbed by 1e-05microM of 0316684-0000					81.4668 0 % 90.73
MCF7 perturbed by 8.6e-06microM of clemastine					83.1166 0.54 % 91.29
MCF7 perturbed by 1.1e-05microM of fenofibrate					82.1433 0.04 % 91.05
MCF7 perturbed by 9.8e-06microM of trazodone					78.2042 0.7 % 88.75
MCF7 perturbed by 1e-05microM of 16-phenyltetranorprostaglandin E2					82.7632 4.13 % 89.32
MCF7 perturbed by 1e-05microM of 11-deoxy-16-16-dimethylprostaglandin E2					79.7293 4.2 % 87.76