Connectivity Map Batch 02 Random Load - CMAP22

From ArrayWiki

PubMed ID:	17008526
Contact:	Justin Lamb Broad Institute of MIT and Harvard
Species:	homo sapiens (cell lines)
Platform:	HT_HG-U133A
Number of Samples:	201
Date Range:	12/31/1969 - 12/31/1969
Experiment Quality:	86.31 0.02 % 93.15
Link to Repository:
Original Intensity Data:	Download
Clean Intensity Data:	Download
Original PLIER Data:	Download
Clean PLIER Data:	Download
Clean CEL Files:	Download
Data Rank:

To pursue a systematic approach to the discovery of functional connections among diseases, genetic perturbation, and drug action, we have created the first installment of a reference collection of gene-expression profiles from cultured human cells treated with bioactive small molecules, together with pattern-matching software to mine these data. We demonstrate that this 'Connectivity Map' resource can be used to find connections among small molecules sharing a mechanism of action, chemicals and physiological processes, and diseases and drugs. These results indicate the feasibility of the approach and suggest the value of a large-scale community Connectivity Map project.

Contents 1 Contributors 2 Protocols 3 References 4 Links 5 Analysis 6 Samples/Quality Control

[edit] Contributors

[edit] Protocols

[edit] References

[edit] Links

[edit] Analysis

Gel Plots

Before Normalization

After Normalization & Cleaning

[edit] Samples/Quality Control

Sample	Date Scanned	Source Link	Probe Variance Data	Artifact Mask	Quality Score
PC3 perturbed by 1e-05microM of LY-294002					88.0341 0.98 % 93.53
vehicle					86.3101 1.58 % 92.37
vehicle					86.5088 2.63 % 91.94
PC3 perturbed by 1e-05microM of prochlorperazine					86.8263 0.02 % 93.4
MCF7 perturbed by 9.4e-06microM of trimethobenzamide					93.3703 0.01 % 96.68
vehicle					93.4192 2.38 % 95.52
MCF7 perturbed by 1.36e-05microM of mebendazole					92.7456 1.65 % 95.55
HL60 perturbed by 1.24e-05microM of gelsemine					90.3681 0.04 % 95.17
HL60 perturbed by 8.2e-06microM of glimepiride					91.5548 0.03 % 95.76
HL60 perturbed by 9.2e-06microM of vitexin					90.9688 0.09 % 95.44
HL60 perturbed by 8e-06microM of diflorasone					91.2573 0.02 % 95.62
HL60 perturbed by 1.7e-05microM of sparteine					91.2278 0.15 % 95.54
HL60 perturbed by 8e-06microM of minocycline					91.4826 1.23 % 95.13
HL60 perturbed by 8.4e-06microM of trifluoperazine					91.056 1.44 % 94.81
vehicle					87.2499 2.47 % 92.39
PC3 perturbed by 1.34e-05microM of disulfiram					86.724 3.19 % 91.77
MCF7 perturbed by 1e-05microM of haloperidol					92.4602 0.01 % 96.22
MCF7 perturbed by 3.2e-06microM of atracurium besilate					92.7779 0 % 96.39
vehicle					93.3301 0.03 % 96.65
MCF7 perturbed by 1.26e-05microM of moxisylyte					92.7826 0.06 % 96.36
HL60 perturbed by 9.2e-06microM of brompheniramine					90.7324 0.46 % 95.13
HL60 perturbed by 1.46e-05microM of 7-aminocephalosporanic acid					90.2748 0.37 % 94.95
HL60 perturbed by 7.6e-06microM of dequalinium chloride					89.8032 2.07 % 93.86
HL60 perturbed by 3e-06microM of cyanocobalamin					90.121 0.37 % 94.87
HL60 perturbed by 1.54e-05microM of carbarsone					90.3152 0.29 % 95.01
vehicle					90.8725 1.45 % 94.71
HL60 perturbed by 1.12e-05microM of thiamphenicol					88.777 0.54 % 94.12
PC3 perturbed by 1.34e-05microM of noretynodrel					87.1862 3.85 % 91.67
PC3 perturbed by 1.6e-05microM of sulfadiazine					89.7482 0.22 % 94.77
HL60 perturbed by 1.12e-05microM of homatropine					90.7686 2.64 % 94.07
HL60 perturbed by 7.4e-06microM of puromycin					89.5847 0.35 % 94.62
HL60 perturbed by 1.66e-05microM of tetramisole					90.5729 0.06 % 95.26
HL60 perturbed by 1.72e-05microM of melatonin					90.7142 0.67 % 95.02
MCF7 perturbed by 1.02e-05microM of picotamide					92.693 1.27 % 95.71
MCF7 perturbed by 9.6e-06microM of spironolactone					92.9713 0.71 % 96.13
MCF7 perturbed by 8.4e-06microM of terfenadine					92.7811 0.29 % 96.25
MCF7 perturbed by 1.34e-05microM of acetylsalicylsalicylic acid					92.7138 0.54 % 96.09
MCF7 perturbed by 7.2e-06microM of antimycin A					93.1406 0.6 % 96.27
MCF7 perturbed by 8.8e-06microM of astemizole					93.1674 1.32 % 95.92
MCF7 perturbed by 5.4e-06microM of ouabain					92.2673 3.27 % 94.5
vehicle					91.7645 0.01 % 95.88
MCF7 perturbed by 7e-06microM of alpha-ergocryptine					90.7555 0.34 % 95.21
vehicle					89.7896 0.03 % 94.88
vehicle					90.6359 3.35 % 93.64
MCF7 perturbed by 2.84e-05microM of tropine					91.404 3.8 % 93.8
MCF7 perturbed by 2.12e-05microM of phensuximide					91.0033 6.16 % 92.42
MCF7 perturbed by 1.04e-05microM of imidurea					90.4994 5.39 % 92.56
vehicle					90.446 7.1 % 91.68
HL60 perturbed by 1e-05microM of rosiglitazone					90.2185 0.13 % 95.05
MCF7 perturbed by 8.4e-06microM of dinoprost					90.7529 0.01 % 95.37
MCF7 perturbed by 8.6e-06microM of cisapride					91.0041 0 % 95.5
MCF7 perturbed by 5.8e-06microM of amiodarone					91.5717 0.32 % 95.62
MCF7 perturbed by 1.22e-05microM of velnacrine					92.1723 0.07 % 96.05
MCF7 perturbed by 1e-05microM of ergocalciferol					92.8465 2.98 % 94.94
MCF7 perturbed by 1.48e-05microM of zaprinast					92.0313 6.12 % 92.96
vehicle					92.7054 0.5 % 96.1
vehicle					90.0986 3.49 % 93.3
PC3 perturbed by 1.18e-05microM of metoclopramide					89.6507 0.71 % 94.47
MCF7 perturbed by 1.04e-05microM of lomefloxacin					86.5511 2.39 % 92.08
MCF7 perturbed by 1e-07microM of trichostatin A					92.3508 0.87 % 95.74
vehicle					92.5584 0.79 % 95.89
MCF7 perturbed by 1.18e-05microM of lisuride					91.7286 0.23 % 95.75
vehicle					91.7002 0.34 % 95.68
vehicle					92.3199 2.55 % 94.88
MCF7 perturbed by 1e-05microM of hexamethonium bromide					92.8801 0.75 % 96.06
MCF7 perturbed by 1.22e-05microM of levobunolol					92.5298 0.22 % 96.15
MCF7 perturbed by 3.5e-05microM of Prestwick-864					92.5913 0 % 96.3
MCF7 perturbed by 7.2e-06microM of cefsulodin					91.1394 0.17 % 95.49
MCF7 perturbed by 2.34e-05microM of metronidazole					92.3581 0.94 % 95.71
MCF7 perturbed by 1.18e-05microM of tenoxicam					91.649 7.18 % 92.24
MCF7 perturbed by 1.44e-05microM of ethambutol					92.9272 0.01 % 96.46
MCF7 perturbed by 1e-07microM of trichostatin A					92.5535 0.86 % 95.85
vehicle					91.4862 1.72 % 94.88
MCF7 perturbed by 2.52e-05microM of citiolone					90.2527 5.87 % 92.19
MCF7 perturbed by 8.6e-06microM of benfotiamine					90.8336 2.71 % 94.06
PC3 perturbed by 9.4e-06microM of mephentermine					90.3533 0 % 95.18
PC3 perturbed by 1e-07microM of trichostatin A					89.1958 2.13 % 93.53
PC3 perturbed by 8.8e-06microM of halcinonide					90.4133 0 % 95.21
PC3 perturbed by 1.46e-05microM of nalidixic acid					88.2937 0.42 % 93.94
HL60 perturbed by 1.64e-05microM of dioxybenzone					90.7713 4.33 % 93.22
HL60 perturbed by 1.34e-05microM of enilconazole					91.0497 0.04 % 95.51
HL60 perturbed by 2.3e-05microM of N-acetyl-L-leucine					91.4829 2.55 % 94.47
HL60 perturbed by 9e-06microM of denatonium benzoate					91.4586 0.03 % 95.71
HL60 perturbed by 1.06e-05microM of ketorolac					91.4227 0.03 % 95.7
HL60 perturbed by 3.6e-06microM of adipiodone					91.0421 0.03 % 95.51
MCF7 perturbed by 1.06e-05microM of lobelanidine					91.8717 0.38 % 95.75
MCF7 perturbed by 1.98e-05microM of tiabendazole					92.4078 1.01 % 95.7
MCF7 perturbed by 1.4e-05microM of probenecid					93.0489 0.36 % 96.35
PC3 perturbed by 7.4e-06microM of piperacillin					91.9351 0.77 % 95.58
PC3 perturbed by 1.18e-05microM of amiprilose					90.9396 1.59 % 94.67
vehicle					91.1501 0.61 % 95.27
PC3 perturbed by 1.36e-05microM of cinchonine					89.4365 0.87 % 94.28
PC3 perturbed by 1.08e-05microM of etofenamate					90.8906 0.29 % 95.3
MCF7 perturbed by 8.4e-06microM of clofazimine					92.4249 2.15 % 95.14
MCF7 perturbed by 6.8e-06microM of kanamycin					93.1771 2.14 % 95.52
MCF7 perturbed by 2.14e-05microM of phenylpropanolamine					93.3998 2.16 % 95.62
MCF7 perturbed by 1.5e-05microM of clonidine					92.8794 7.31 % 92.78
PC3 perturbed by 1e-05microM of lumicolchicine					90.864 1.41 % 94.73
PC3 perturbed by 1.02e-05microM of corynanthine					90.1507 7.72 % 91.22
HL60 perturbed by 6.6e-06microM of reserpine					91.2574 6.7 % 92.28
MCF7 perturbed by 1e-07microM of trichostatin A					91.2446 0.12 % 95.56
MCF7 perturbed by 1.4e-05microM of acacetin					90.4103 0.2 % 95.11
MCF7 perturbed by 1.7e-05microM of phenelzine					92.0352 0 % 96.02
MCF7 perturbed by 1.24e-05microM of promazine					90.8135 0.19 % 95.31
HL60 perturbed by 9.2e-06microM of zuclopenthixol					90.8257 3.09 % 93.87
HL60 perturbed by 8.8e-06microM of levocabastine					91.4137 3.75 % 93.83
MCF7 perturbed by 1.86e-05microM of sulfaguanidine					92.8003 0 % 96.4
MCF7 perturbed by 1.42e-05microM of hydroxyachillin					91.9839 2.39 % 94.8
vehicle					91.2838 1.84 % 94.72
PC3 perturbed by 2.6e-06microM of vancomycin					89.7513 5.64 % 92.06
vehicle					91.119 1.26 % 94.93
PC3 perturbed by 1.7e-05microM of phenelzine					91.2009 0.69 % 95.26
PC3 perturbed by 1e-07microM of trichostatin A					91.4609 0.4 % 95.53
MCF7 perturbed by 1e-06microM of CP-690334-01					92.1052 0.35 % 95.88
MCF7 perturbed by 7e-06microM of alpha-ergocryptine					92.7182 0.39 % 96.16
MCF7 perturbed by 1.66e-05microM of tetramisole					93.2728 0 % 96.63
MCF7 perturbed by 1.44e-05microM of flutamide					91.8369 0 % 95.92
MCF7 perturbed by 6.8e-06microM of pentamidine					93.1081 0.01 % 96.55
PC3 perturbed by 9.2e-06microM of timolol					91.9784 0.25 % 95.87
MCF7 perturbed by 1.66e-05microM of phenformin					92.7666 0.5 % 96.13
PC3 perturbed by 1e-05microM of fluphenazine					92.297 0.03 % 96.13
PC3 perturbed by 1.12e-05microM of napelline					92.5186 0.49 % 96.01
PC3 perturbed by 5.8e-06microM of meclocycline					92.4089 0.01 % 96.2
PC3 perturbed by 5e-06microM of protoveratrine A					92.0741 0.03 % 96.02
PC3 perturbed by 8.8e-06microM of methylergometrine					91.0429 0.21 % 95.41
PC3 perturbed by 1.58e-05microM of tacrine					90.9466 1.55 % 94.7
PC3 perturbed by 5.4e-06microM of erythromycin					90.6516 2.96 % 93.85
vehicle					90.8365 2.58 % 94.13
PC3 perturbed by 1.02e-05microM of betamethasone					90.7931 1.4 % 94.7
MCF7 perturbed by 1.08e-05microM of isradipine					93.5337 0.17 % 96.68
MCF7 perturbed by 2.14e-05microM of carbimazole					91.9117 0.03 % 95.94
MCF7 perturbed by 1.76e-05microM of nabumetone					92.4881 0.03 % 96.23
PC3 perturbed by 9e-06microM of folic acid					91.2283 0.02 % 95.6
PC3 perturbed by 1e-07microM of trichostatin A					90.5298 2.48 % 94.02
MCF7 perturbed by 1.02e-05microM of perhexiline					90.9519 3.59 % 93.68
MCF7 perturbed by 9.6e-06microM of isoflupredone					91.1349 0.52 % 95.31
MCF7 perturbed by 1.02e-05microM of diethylcarbamazine					91.9186 0.42 % 95.75
MCF7 perturbed by 8.4e-06microM of flucloxacillin					91.8395 0.33 % 95.76
PC3 perturbed by 1.22e-05microM of ondansetron					92.3117 0.47 % 95.92
PC3 perturbed by 1.32e-05microM of mianserin					92.4467 0.7 % 95.88
PC3 perturbed by 2.44e-05microM of ricinine					90.7016 0.16 % 95.27
PC3 perturbed by 1.34e-05microM of hexylcaine					91.3442 0.27 % 95.54
MCF7 perturbed by 6.8e-06microM of tonzonium bromide					92.0686 0.4 % 95.83
MCF7 perturbed by 1e-06microM of trichostatin A					91.9176 3.84 % 94.04
MCF7 perturbed by 1e-05microM of PF-00562151-00					93.2815 0.87 % 96.21
MCF7 perturbed by 1e-05microM of LY-294002					91.468 3.04 % 94.21
MCF7 perturbed by 1.28e-05microM of clenbuterol					92.8714 1.18 % 95.84
MCF7 perturbed by 6e-06microM of cephaeline					90.9833 0.03 % 95.47
MCF7 perturbed by 1.36e-05microM of vinburnine					90.488 2.93 % 93.78
MCF7 perturbed by 1.18e-05microM of isocorydine					89.7092 3.05 % 93.33
MCF7 perturbed by 1.08e-05microM of berberine					90.6695 4.29 % 93.19
MCF7 perturbed by 1.02e-05microM of picotamide					91.5983 2.02 % 94.79
MCF7 perturbed by 1.86e-05microM of kinetin					92.0238 0 % 96.01
MCF7 perturbed by 6.2e-06microM of physostigmine					93.5637 0.1 % 96.73
vehicle					93.7166 2.85 % 95.43
MCF7 perturbed by 9.6e-06microM of spironolactone					93.3296 3.12 % 95.1
MCF7 perturbed by 1.86e-05microM of etanidazole					93.5247 0.98 % 96.27
MCF7 perturbed by 2.04e-05microM of methacholine chloride					93.4014 5 % 94.2
HL60 perturbed by 2.4e-05microM of oxedrine					89.2781 3.39 % 92.95
HL60 perturbed by 1e-05microM of 15-delta prostaglandin J2					88.7403 3.45 % 92.64
HL60 perturbed by 1e-05microM of troglitazone					90.1814 4.87 % 92.65
HL60 perturbed by 3.92e-05microM of cycloserine					90.0266 3.66 % 93.18
HL60 perturbed by 1.62e-05microM of Prestwick-1084					88.7201 3.7 % 92.51
PC3 perturbed by 1.16e-05microM of clopamide					91.0892 3.48 % 93.81
PC3 perturbed by 1.34e-05microM of protriptyline					90.6477 3.99 % 93.33
PC3 perturbed by 2.38e-05microM of zoxazolamine					90.9429 4.09 % 93.42
PC3 perturbed by 1.46e-05microM of procaine					91.159 3.56 % 93.8
MCF7 perturbed by 1e-05microM of AG-012559					78.9714 3.47 % 87.75
vehicle					92.4337 0.01 % 96.21
MCF7 perturbed by 5e-05microM of 5707885					93.077 0.02 % 96.53
MCF7 perturbed by 1e-05microM of Prestwick-675					92.9626 0.03 % 96.47
vehicle					92.6427 1.05 % 95.8
MCF7 perturbed by 1e-08microM of fulvestrant					83.9441 1.66 % 91.14
MCF7 perturbed by 1e-05microM of clozapine					85.4359 1.39 % 92.02
MCF7 perturbed by 1.06e-05microM of clorsulon					86.0599 2.12 % 91.97
vehicle					81.7432 2.59 % 89.58
MCF7 perturbed by 7.6e-06microM of deptropine					92.5989 0.08 % 96.26
MCF7 perturbed by 1.76e-05microM of oxybenzone					91.6024 0.06 % 95.77
MCF7 perturbed by 1.68e-05microM of proxyphylline					91.9931 0.02 % 95.99
MCF7 perturbed by 2.1e-05microM of octopamine					91.8572 0.43 % 95.71
MCF7 perturbed by 1.34e-05microM of adrenosterone					91.992 0.24 % 95.87
PC3 perturbed by 5e-07microM of 6-bromoindirubin-3'-oxime					90.8222 3.54 % 93.64
vehicle					86.0804 2.28 % 91.9
MCF7 perturbed by 1.42e-05microM of sulfamonomethoxine					86.7288 0.27 % 93.23
vehicle					85.3261 0.07 % 92.63
PC3 perturbed by 1.34e-05microM of fenspiride					81.3979 1.7 % 89.85
PC3 perturbed by 7.8e-06microM of chloroquine					82.7489 1.78 % 90.49
PC3 perturbed by 8.4e-06microM of cefazolin					74.8563 1.69 % 86.58
vehicle					77.1088 1.03 % 88.04
MCF7 perturbed by 8.4e-06microM of metacycline					76.0865 0.37 % 87.86
PC3 perturbed by 1e-06microM of SB-202190					79.1981 0.21 % 89.49
PC3 perturbed by 1e-06microM of SB-203580					77.4124 2.72 % 87.35
PC3 perturbed by 1e-06microM of AH-6809					76.9094 0 % 88.45
vehicle					81.1151 0.01 % 90.55
MCF7 perturbed by 1e-05microM of AR-A014418					78.1044 0.27 % 88.92
MCF7 perturbed by 1e-07microM of thapsigargin					80.1949 0 % 90.1
MCF7 perturbed by 1.62e-05microM of chlorphenesin					80.5923 0.42 % 90.09
MCF7 perturbed by 1.1e-05microM of cortisone					78.3357 0.73 % 88.8
MCF7 perturbed by 1.04e-05microM of homochlorcyclizine					78.1471 0.58 % 88.78
MCF7 perturbed by 1e-05microM of 15(S)-15-methylprostaglandin E2					84.0636 2.55 % 90.76
MCF7 perturbed by 1e-05microM of 15(S)-15-methylprostaglandin E2					80.249 3.82 % 88.21