Connectivity Map Batch 02 Random Load - CMAP12

From ArrayWiki

PubMed ID:	17008526
Contact:	Justin Lamb Broad Institute of MIT and Harvard
Species:	homo sapiens (cell lines)
Platform:	HT_HG-U133A
Number of Samples:	201
Date Range:	12/31/1969 - 12/31/1969
Experiment Quality:	92.78 0.07 % 96.35
Link to Repository:
Original Intensity Data:	Download
Clean Intensity Data:	Download
Original PLIER Data:	Download
Clean PLIER Data:	Download
Clean CEL Files:	Download
Data Rank:

To pursue a systematic approach to the discovery of functional connections among diseases, genetic perturbation, and drug action, we have created the first installment of a reference collection of gene-expression profiles from cultured human cells treated with bioactive small molecules, together with pattern-matching software to mine these data. We demonstrate that this 'Connectivity Map' resource can be used to find connections among small molecules sharing a mechanism of action, chemicals and physiological processes, and diseases and drugs. These results indicate the feasibility of the approach and suggest the value of a large-scale community Connectivity Map project.

Contents 1 Contributors 2 Protocols 3 References 4 Links 5 Analysis 6 Samples/Quality Control

[edit] Contributors

[edit] Protocols

[edit] References

[edit] Links

[edit] Analysis

Gel Plots

Before Normalization

After Normalization & Cleaning

[edit] Samples/Quality Control

Sample	Date Scanned	Source Link	Probe Variance Data	Artifact Mask	Quality Score
PC3 perturbed by 1e-08microM of wortmannin					88.4533 0.81 % 93.82
MCF7 perturbed by 1.58e-05microM of cimetidine					92.783 0.04 % 96.37
HL60 perturbed by 1.28e-05microM of dydrogesterone					90.2284 0.08 % 95.07
vehicle					91.2991 0.07 % 95.61
vehicle					90.7753 5.34 % 92.72
HL60 perturbed by 1.1e-05microM of clemizole					91.22 0.1 % 95.56
HL60 perturbed by 8.4e-06microM of ifenprodil					91.6279 0.04 % 95.79
HL60 perturbed by 9.4e-06microM of flavoxate					91.2861 0.14 % 95.57
PC3 perturbed by 4.4e-06microM of gallamine triethiodide					87.8989 3.17 % 92.36
PC3 perturbed by 2.36e-05microM of tranylcypromine					88.071 3.57 % 92.25
MCF7 perturbed by 1e-05microM of LY-294002					91.7557 0.31 % 95.72
HL60 perturbed by 6.6e-06microM of prochlorperazine					90.0346 1.57 % 94.23
HL60 perturbed by 1.1e-05microM of bezafibrate					89.4967 1.05 % 94.22
vehicle					89.6907 0.37 % 94.66
vehicle					88.49 0.12 % 94.18
PC3 perturbed by 1.24e-05microM of chloramphenicol					90.6873 0.23 % 95.23
HL60 perturbed by 1.5e-05microM of ciclopirox					89.5588 0.09 % 94.74
HL60 perturbed by 2.4e-05microM of ethionamide					89.9318 0.03 % 94.95
HL60 perturbed by 1.54e-05microM of norcyclobenzaprine					90.4131 0.4 % 95.01
HL60 perturbed by 2.24e-05microM of phenacetin					89.7375 1.17 % 94.28
vehicle					90.5179 1.44 % 94.54
MCF7 perturbed by 2.54e-05microM of tranexamic acid					92.6912 2.37 % 95.16
MCF7 perturbed by 1.38e-05microM of hyoscyamine					93.1218 1.73 % 95.7
HL60 perturbed by 5e-06microM of merbromin					88.7788 0.73 % 94.03
vehicle					90.9555 4.15 % 93.4
vehicle					90.8241 0.86 % 94.98
HL60 perturbed by 9e-06microM of benzethonium chloride					87.7068 3.34 % 92.18
MCF7 perturbed by 1.06e-05microM of droperidol					92.4457 0.07 % 96.19
MCF7 perturbed by 1.58e-05microM of tacrine					91.7447 0.34 % 95.7
MCF7 perturbed by 5.4e-06microM of alimemazine					90.6313 0.02 % 95.31
MCF7 perturbed by 7.8e-06microM of cromoglicic acid					91.3512 0.33 % 95.51
MCF7 perturbed by 9.4e-06microM of mephentermine					89.8412 0.15 % 94.84
vehicle					89.0355 0.05 % 94.49
MCF7 perturbed by 2.18e-05microM of corbadrine					90.7215 0 % 95.36
MCF7 perturbed by 5.8e-06microM of penbutolol					90.1924 4.32 % 92.94
MCF7 perturbed by 2.8e-06microM of verteporfin					89.8673 4.63 % 92.62
HL60 perturbed by 2.4e-05microM of pyrithyldione					90.081 6.22 % 91.93
HL60 perturbed by 1e-05microM of fluphenazine					90.4853 2.78 % 93.85
vehicle					88.4729 4.12 % 92.18
HL60 perturbed by 9.8e-06microM of carbinoxamine					88.906 0.64 % 94.13
HL60 perturbed by 1e-07microM of monorden					90.0769 0.72 % 94.68
HL60 perturbed by 1.52e-05microM of cinoxacin					89.0882 3.81 % 92.64
MCF7 perturbed by 1.4e-05microM of piperine					89.3957 0.15 % 94.62
PC3 perturbed by 1e-06microM of 0179445-0000					89.3442 1.58 % 93.88
PC3 perturbed by 1.18e-05microM of dicoumarol					89.4521 0.11 % 94.67
MCF7 perturbed by 1.08e-05microM of finasteride					86.9622 1.47 % 92.75
MCF7 perturbed by 1e-07microM of trichostatin A					85.1093 40.39 % 72.36
MCF7 perturbed by 1.56e-05microM of prilocaine					88.7929 1.32 % 93.74
MCF7 perturbed by 9.8e-06microM of ipratropium bromide					91.4916 0.9 % 95.3
MCF7 perturbed by 1.7e-05microM of sparteine					90.8633 0.04 % 95.41
MCF7 perturbed by 1e-05microM of genistein					91.2784 1.38 % 94.95
vehicle					91.3655 0.26 % 95.55
MCF7 perturbed by 1e-07microM of sirolimus					91.911 0.93 % 95.49
MCF7 perturbed by 5e-05microM of valproic acid					91.5962 0.73 % 95.43
MCF7 perturbed by 1e-06microM of nordihydroguaiaretic acid					92.5174 0.98 % 95.77
MCF7 perturbed by 1.18e-05microM of (-)-MK-801					92.2622 3.84 % 94.21
MCF7 perturbed by 8.6e-06microM of sulconazole					92.307 0.73 % 95.79
MCF7 perturbed by 1.04e-05microM of colecalciferol					91.0226 0.53 % 95.25
MCF7 perturbed by 1.28e-05microM of amoxapine					91.5821 0.91 % 95.34
vehicle					92.1478 0.82 % 95.66
MCF7 perturbed by 1.48e-05microM of arcaine					92.2476 0.11 % 96.07
MCF7 perturbed by 1.38e-05microM of piromidic acid					92.6583 0.18 % 96.24
MCF7 perturbed by 1.3e-05microM of sotalol					91.7515 2.37 % 94.69
MCF7 perturbed by 1.32e-05microM of amiloride					90.0473 4.26 % 92.89
MCF7 perturbed by 1e-07microM of trichostatin A					89.3991 3.39 % 93
MCF7 perturbed by 1.18e-05microM of dicoumarol					88.6988 5.68 % 91.51
vehicle					89.9423 0 % 94.97
PC3 perturbed by 1.36e-05microM of meclofenoxate					89.583 3.72 % 92.93
HL60 perturbed by 1.64e-05microM of flurbiprofen					90.8057 0.02 % 95.39
HL60 perturbed by 2.1e-05microM of octopamine					90.8158 0.03 % 95.39
MCF7 perturbed by 1.18e-05microM of tenoxicam					91.5185 1.4 % 95.06
MCF7 perturbed by 2.8e-05microM of trimethadione					91.2191 0.91 % 95.15
PC3 perturbed by 1.18e-05microM of chlorcyclizine					91.461 3.47 % 94
PC3 perturbed by 1.18e-05microM of dicoumarol					91.9713 1.42 % 95.27
PC3 perturbed by 1.06e-05microM of famprofazone					91.2118 2.31 % 94.45
PC3 perturbed by 1.86e-05microM of memantine					90.4472 2.79 % 93.83
PC3 perturbed by 1.64e-05microM of azacitidine					90.3328 0.62 % 94.85
PC3 perturbed by 1.54e-05microM of tiaprofenic acid					91.083 1.62 % 94.73
PC3 perturbed by 1.22e-05microM of boldine					89.2053 1.72 % 93.74
MCF7 perturbed by 9.6e-06microM of ambroxol					91.1274 3.54 % 93.79
MCF7 perturbed by 7.8e-06microM of nicardipine					92.5537 2.09 % 95.23
PC3 perturbed by 1.46e-05microM of nifuroxazide					90.4764 2.44 % 94.02
PC3 perturbed by 1.06e-05microM of cyclopenthiazide					87.3365 11.96 % 87.69
vehicle					90.3223 8.67 % 90.83
HL60 perturbed by 6.6e-06microM of carbenoxolone					91.0629 2.13 % 94.47
HL60 perturbed by 9e-06microM of propantheline bromide					90.6903 6.37 % 92.16
HL60 perturbed by 1.06e-05microM of trichlormethiazide					90.5494 4.59 % 92.98
HL60 perturbed by 1.56e-05microM of quinpirole					90.1811 0.43 % 94.88
HL60 perturbed by 1.9e-05microM of zalcitabine					90.492 0.28 % 95.1
HL60 perturbed by 1.32e-05microM of spaglumic acid					90.5799 0.59 % 95
HL60 perturbed by 8.6e-06microM of moracizine					90.8398 0.02 % 95.41
MCF7 perturbed by 9.6e-06microM of gabexate					88.0662 1.17 % 93.45
MCF7 perturbed by 1.08e-05microM of minaprine					91.3306 1.53 % 94.9
PC3 perturbed by 7.4e-06microM of levopropoxyphene					90.6038 2.03 % 94.28
vehicle					90.7461 2.28 % 94.23
PC3 perturbed by 8.2e-06microM of prednicarbate					89.8565 2.68 % 93.59
vehicle					90.9223 2.78 % 94.07
vehicle					90.5731 2.49 % 94.04
PC3 perturbed by 1.22e-05microM of chloropyramine					91.2344 0.97 % 95.13
PC3 perturbed by 1.38e-05microM of piromidic acid					90.3335 3.12 % 93.61
vehicle					91.0253 0.88 % 95.07
MCF7 perturbed by 1e-06microM of CP-320650-01					90.8914 0.02 % 95.43
MCF7 perturbed by 1.96e-05microM of ethotoin					92.2067 0 % 96.1
MCF7 perturbed by 6.4e-06microM of novobiocin					92.0848 0 % 96.04
MCF7 perturbed by 3.92e-05microM of levcycloserine					90.0846 0.08 % 95
MCF7 perturbed by 1e-07microM of trichostatin A					90.561 0.12 % 95.22
PC3 perturbed by 2.26e-05microM of cotinine					91.4284 2.65 % 94.39
MCF7 perturbed by 1.46e-05microM of nalidixic acid					91.8587 1.48 % 95.19
PC3 perturbed by 1.14e-05microM of clomipramine					90.6366 1.28 % 94.68
PC3 perturbed by 1.62e-05microM of methoxamine					91.0403 0.45 % 95.29
vehicle					91.4341 3.35 % 94.04
vehicle					91.0639 2.07 % 94.5
PC3 perturbed by 6.6e-06microM of hesperidin					90.9428 1.22 % 94.86
vehicle					91.3922 2.72 % 94.34
PC3 perturbed by 9.2e-06microM of ethaverine					91.4567 1.86 % 94.8
MCF7 perturbed by 1.28e-05microM of amitriptyline					92.7593 0.35 % 96.2
MCF7 perturbed by 1.2e-05microM of nizatidine					92.1009 0.2 % 95.95
MCF7 perturbed by 1.36e-05microM of S-propranolol					90.0852 0.01 % 95.04
PC3 perturbed by 1e-05microM of bambuterol					90.5812 0.92 % 94.83
PC3 perturbed by 1.22e-05microM of azaperone					91.0084 0.83 % 95.09
vehicle					91.2328 1.09 % 95.07
MCF7 perturbed by 9e-06microM of denatonium benzoate					91.5611 0.85 % 95.35
MCF7 perturbed by 1.34e-05microM of norethisterone					91.7383 0.01 % 95.86
MCF7 perturbed by 1.54e-05microM of oxolinic acid					90.4324 2.19 % 94.12
MCF7 perturbed by 3.6e-06microM of adipiodone					91.7239 1.59 % 95.07
vehicle					91.2199 0.18 % 95.52
vehicle					92.1591 0.03 % 96.06
PC3 perturbed by 4.4e-06microM of gallamine triethiodide					91.6638 0.01 % 95.83
PC3 perturbed by 1.14e-05microM of tetrahydroalstonine					90.6963 0.46 % 95.12
PC3 perturbed by 8e-06microM of oxytetracycline					92.4243 0.37 % 96.03
MCF7 perturbed by 1.38e-05microM of androsterone					87.4168 2.31 % 92.55
MCF7 perturbed by 1.38e-05microM of epiandrosterone					91.0198 0.79 % 95.11
MCF7 perturbed by 1.16e-05microM of betaxolol					90.019 1.61 % 94.2
MCF7 perturbed by 1e-05microM of haloperidol					90.9453 0.01 % 95.47
vehicle					91.6217 0.23 % 95.7
MCF7 perturbed by 1e-06microM of geldanamycin					90.2111 3.78 % 93.21
MCF7 perturbed by 1e-06microM of tretinoin					91.3908 0.05 % 95.67
MCF7 perturbed by 2.06e-05microM of butyl hydroxybenzoate					89.6671 0.44 % 94.61
MCF7 perturbed by 1.24e-05microM of oxyphenbutazone					90.1196 1.29 % 94.41
MCF7 perturbed by 1.12e-05microM of benzylpenicillin					92.1642 0.3 % 95.93
MCF7 perturbed by 3e-06microM of dihydroergotamine					89.8634 1.21 % 94.32
MCF7 perturbed by 9.6e-06microM of ramipril					90.5559 4.4 % 93.08
vehicle					91.1727 0.15 % 95.51
MCF7 perturbed by 2.32e-05microM of sulfanilamide					90.6173 0.02 % 95.3
MCF7 perturbed by 1.18e-05microM of atropine oxide					91.6007 0.02 % 95.79
MCF7 perturbed by 1.58e-05microM of triamterene					91.9939 0.01 % 95.99
MCF7 perturbed by 1.54e-05microM of thalidomide					92.4237 0.46 % 95.98
MCF7 perturbed by 1.8e-05microM of phenindione					92.5661 0 % 96.28
MCF7 perturbed by 2.8e-06microM of dihydrostreptomycin					92.4599 4.36 % 94.05
MCF7 perturbed by 2.1e-05microM of cytisine					92.8944 0.2 % 96.35
HL60 perturbed by 1e-07microM of trichostatin A					87.9476 0.21 % 93.87
PC3 perturbed by 8.8e-06microM of Prestwick-1083					90.5467 3.31 % 93.62
PC3 perturbed by 2.24e-05microM of ascorbic acid					90.648 3.73 % 93.46
PC3 perturbed by 9.6e-06microM of aminophylline					90.477 3.53 % 93.47
PC3 perturbed by 1e-05microM of AG-012559					76.5794 3.16 % 86.71
vehicle					81.0973 2.44 % 89.33
vehicle					92.4638 3.31 % 94.58
PC3 perturbed by 1e-05microM of PF-00539758-00					92.0468 0.03 % 96.01
MCF7 perturbed by 1e-05microM of AG-013608					92.2327 2.09 % 95.07
MCF7 perturbed by 9.6e-06microM of podophyllotoxin					90.3496 0.04 % 95.15
vehicle					91.5766 0.18 % 95.7
MCF7 perturbed by 8.6e-06microM of clobetasol					92.3405 0.04 % 96.15
MCF7 perturbed by 1.28e-05microM of heliotrine					92.7219 0 % 96.36
MCF7 perturbed by 2.44e-05microM of ricinine					91.3956 0.01 % 95.69
MCF7 perturbed by 1e-07microM of sirolimus					83.84 1.1 % 91.37
MCF7 perturbed by 5.2e-06microM of viomycin					83.2857 2.17 % 90.56
MCF7 perturbed by 1.18e-05microM of meclofenamic acid					84.7299 1.71 % 91.51
MCF7 perturbed by 1.3e-05microM of nimesulide					81.9955 3.07 % 89.46
MCF7 perturbed by 1.28e-05microM of triprolidine					80.18 0.25 % 89.97
MCF7 perturbed by 4.2e-06microM of asiaticoside					82.1349 0.2 % 90.97
MCF7 perturbed by 1.34e-05microM of enilconazole					91.2796 0.87 % 95.2
MCF7 perturbed by 9.4e-06microM of pizotifen					92.0312 0.5 % 95.77
MCF7 perturbed by 8.4e-06microM of fluspirilene					91.3907 0.85 % 95.27
MCF7 perturbed by 1.18e-05microM of terguride					91.8373 0.86 % 95.49
PC3 perturbed by 2e-05microM of 5194442					90.9566 3.2 % 93.88
MCF7 perturbed by 1e-05microM of PHA-00665752					90.6263 3.94 % 93.34
MCF7 perturbed by 1e-06microM of geldanamycin					80.2554 3.94 % 88.16
MCF7 perturbed by 1e-07microM of estradiol					83.8916 2.6 % 90.64
MCF7 perturbed by 1e-08microM of alpha-estradiol					80.228 1.83 % 89.2
MCF7 perturbed by 9.6e-06microM of podophyllotoxin					85.178 0.24 % 92.47
MCF7 perturbed by 2.6e-06microM of gentamicin					84.6559 1.1 % 91.78
MCF7 perturbed by 9.2e-06microM of hecogenin					81.3149 1.58 % 89.87
PC3 perturbed by 1.34e-05microM of azapropazone					83.9722 1.15 % 91.41
PC3 perturbed by 1.96e-05microM of Prestwick-1103					84.4172 1.61 % 91.4
PC3 perturbed by 1.02e-05microM of chenodeoxycholic acid					84.2163 0.18 % 92.02
PC3 perturbed by 1.68e-05microM of proxyphylline					80.6462 0.64 % 90
PC3 perturbed by 1.02e-05microM of ursodeoxycholic acid					84.0176 0.57 % 91.73
PC3 perturbed by 1.2e-05microM of propoxycaine					77.4512 1.47 % 87.99
vehicle					76.1621 0.41 % 87.87
MCF7 perturbed by 9.2e-06microM of fursultiamine					76.9324 0.91 % 88.01
PC3 perturbed by 1.72e-05microM of isocarboxazid					73.7131 1.3 % 86.2
PC3 perturbed by 1.68e-05microM of nitrofurantoin					74.0229 4.38 % 84.82
MCF7 perturbed by 1.48e-05microM of meptazinol					76.8784 0.25 % 88.31
MCF7 perturbed by 8e-06microM of minocycline					80.2122 0.45 % 89.88
MCF7 perturbed by 1.1e-05microM of fenofibrate					81.6387 0.03 % 90.81
MCF7 perturbed by 1.32e-05microM of tetracaine					79.4056 1.32 % 89.04
MCF7 perturbed by 1.66e-05microM of methocarbamol					78.4597 1.23 % 88.62
MCF7 perturbed by 2.62e-05microM of 8-azaguanine					80.9421 0.02 % 90.46
vehicle					79.0625 5.31 % 86.87
PC3 perturbed by 1e-05microM of suramin sodium					78.2441 3.59 % 87.33
PC3 perturbed by 1e-06microM of IC-86621					77.3114 7.32 % 84.99